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Alexa Fluor® 488 anti-rat IgG2a Antibody

Pricing & Availability
Clone
MRG2a-83 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G2a
Isotype
Mouse IgG
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Product Citations
publications
MRG2a-83_A488_IgG2a_Antibody_1_012521
C57BL/6 mouse splenocytes were stained with purified anti-mouse CD19 followed by anti-rat IgG2a (clone MRG2a-83) Alexa Fluor® 488
  • MRG2a-83_A488_IgG2a_Antibody_1_012521
    C57BL/6 mouse splenocytes were stained with purified anti-mouse CD19 followed by anti-rat IgG2a (clone MRG2a-83) Alexa Fluor® 488
  • MRG2a-83_A488_IgG2a_Antibody_2_012521
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 5 µg/mL of purified anti-mouse/human CD45R/B220 overnight at 4°C followed by 2.5 µg/mL of anti-rat IgG2a (clone MRG2a-83) Alexa Fluor® 488 (red) and 2.5 µg/mL anti-mouse CD3 Alexa Fluor® 594 (green) for 2 hours at room temperature. The image was captured by 10X objective. Nuclei were counter stained with DAPI. The image was captured by 10X objective.
Compare all formats See Alexa Fluor® 488 spectral data
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407513 25 µg 72€
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407514 100 µg 156€
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Description

The MRG2a-83 monoclonal antibody reacts with rat immunoglobulin G2a (IgG2a) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). It does not react with other isotypes. The MRG2a-83 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mixed rat Igs
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC – Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application References
  1. Inoue S, et al. 2006. Cancer Research 66:7741.
RRID
AB_2910464 (BioLegend Cat. No. 407513)
AB_2910464 (BioLegend Cat. No. 407514)

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Go To Top Version: 1    Revision Date: 01/25/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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