Alexa Fluor 647 anti-Acetylated Lysine Antibody anti-Acetylated Lysine

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Clone: 15G10 (See other available formats)
Regulatory Status: RUO
Isotype: Mouse IgG2b, κ
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Product Citations: publications

15G10_AF647_acetylated_lysine_Antibody_ICFC_1_031815 — Human cervical carcinoma cell line, HeLa, was incubated with (top) or without (bottom) Trichostatin A (100 ng/ml, overnight), then treated with FOXP3 Fix/Perm Buffer Set. Cells were then stained with anti-acetylated lysine (clone 15G10) Alexa Fluor® 647 (filled histogram) or mouse IgG2b, κ Alexa Fluor® 647 isotype control (open histogram).

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623405

25 tests

128€

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623406

100 tests

296€

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Description

Proteins are reversibly and dynamically acetylated on the ε-amino group of lysine by acetyltransferases and deacetylated by deacetylases. This post-translational modification can regulate protein function (interactions with other proteins and DNA binding). Histones and transcription factors (PCAF, p53, p300, etc) appear to be the major targets of acetyltransferases. Acetylation is usually associated with chromatin remodeling and transcriptional activation, although in some cases (telomeres) it is associated with gene silencing. The 15G10 antibody recognizes acetylated lysine residues on proteins.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Acetylated protein mixture
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
RRID: AB_2565505 (BioLegend Cat. No. 623405)
AB_2565505 (BioLegend Cat. No. 623406)

Antigen Details

Structure: Proteins are acetylated by the reversible transfer of acetyl-CoA to the ε-amino group of lysine
Function: Post-translational modification of proteins by acetyltransferases and deacetylated by deacetylases to regulate protein-protein and protein-DNA interactions
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References: 1. Wang S, et al. 2004. Novartis Found. Symp. 259:238.
2. Legube G, et al. 2003. EMBO Rep. 4:944.
3. Chen LF, et al. 2003. J. Mol. Med. 81:549.
Gene ID: 8850 View all products for this Gene ID
UniProt: View information about Acetylated Lysine on UniProt.org

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Other Formats

View All Acetylated Lysine Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Acetylated Lysine	15G10	WB,ICC
PE anti-Acetylated Lysine	15G10	ICFC
Alexa Fluor® 647 anti-Acetylated Lysine	15G10	ICFC
PE/Cyanine7 anti-Acetylated Lysine	15G10	ICFC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

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