- Clone
- W19258A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Avi-Tag, AviTag, Avi epitope Tag
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Avi-tag is a 15 amino acid sequence, and the minimal acceptor peptide of a larger protein domain that serves as a substrate for the biotin ligase BirA from E. coli. Although one of the major advantages of Avi-tag fusion proteins is a controlled biotinylation in this domain, Avi-tag is also a useful tool in cell and molecular biology.
Product DetailsProduct Details
- Verified Reactivity
- Epitope tag
- Reported Reactivity
- Species independent
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
-
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) - Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC (test sizes) - Quality tested
ICC (µg size) - Quality tested - Recommended Usage
-
Each lot of the test-sized antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
Each lot of the µg-sized antibody is quality control tested by immunocytochemistry. For immunocytochemistry using the ug size, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
This clone recognizes proteins tagged with Avi-tag sequence irrespective of its biotinylation status.
- Additional Product Notes
-
ICFC: Cells were fixed and permeabilizes using Cyto -Fast™ Buffer system
ICC: Cells were fixed with 4% PFA and permeabilized with Triton X-100 - RRID
-
AB_3068042 (BioLegend Cat. No. 606759)
AB_3068042 (BioLegend Cat. No. 606762)
AB_3068042 (BioLegend Cat. No. 606760)
Antigen Details
- Structure
- GLNDIFEAQKIEWHE. Avi-tag amino acid sequence
- Distribution
-
Depends on the subcellular location of the tagged protein
- Biology Area
- Cell Biology, Protein Purification, Protein Trafficking and Clearance
- Gene ID
- NA
- UniProt
- View information about Avi-tag on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Avi-tag Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Avi-tag | W19258A | WB,ICC,ICFC,IP |
Alexa Fluor® 488 anti-Avi-tag | W19258A | ICFC,ICC |
Alexa Fluor® 594 anti-Avi-tag | W19258A | ICC |
Alexa Fluor® 647 anti-Avi-tag | W19258A | ICFC,ICC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Avi-tag
Whole cell extracts (15 µg total protein/lane) from HEL cell... Whole cell extracts (300 µg) total protein prepared from unt... CHO cells untransfected or transfected with an expression ve... CHO cells transfected with an expression vector for VEGF-165... -
Alexa Fluor® 488 anti-Avi-tag
CHO cells untransfected (negative control, left panel) or ex... CHO cells untransfected (left panel) or transfected (right p... -
Alexa Fluor® 594 anti-Avi-tag
CHO cells untransfected (left panel) or transfected (right p... -
Alexa Fluor® 647 anti-Avi-tag
CHO cells untransfected (negative control, left panel) or ex... CHO cells untransfected (left panel) or transfected (right p...
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