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Alexa Fluor® 700 anti-human CD20 Antibody

Pricing & Availability
Clone
S18015E (See other available formats)
Regulatory Status
RUO
Other Names
B1, Bp35
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
S18015E_AlexaFluor700_CD20_Antibody_073123
Human peripheral blood lymphocytes were stained with anti-human CD19 Brilliant Violet 421™ and anti-human CD20 (clone S18015E) Alexa Fluor® 700 (left) or mouse IgG2a, κ Alexa Fluor® 700 isotype control (right).
  • S18015E_AlexaFluor700_CD20_Antibody_073123
    Human peripheral blood lymphocytes were stained with anti-human CD19 Brilliant Violet 421™ and anti-human CD20 (clone S18015E) Alexa Fluor® 700 (left) or mouse IgG2a, κ Alexa Fluor® 700 isotype control (right).
Compare all formats See Alexa Fluor® 700 spectral data
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375523 25 tests 124€
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375524 100 tests 252€
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Description

CD20 (also known as B1 and Bp35) is a four transmembrane spanning protein measuring 33-37 kD. Expressed on pre-B cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. Involved in B-cell activation and is associated with a variety of Src family kinases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD20 transfectant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
RRID
AB_3068239 (BioLegend Cat. No. 375523)
AB_3068239 (BioLegend Cat. No. 375524)

Antigen Details

Structure
Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
Distribution

B cell, T cell subsets

Function
B cell activation
Interaction
The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn).
Ligand/Receptor
Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Cell Type
B cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Hultin LE, et al. 1993. Cytometry 14:196.
  2. Tedder T and Engel P. 1994. Immunol. Today 15:450.
Gene ID
931 View all products for this Gene ID
UniProt
View information about CD20 on UniProt.org
Go To Top Version: 1    Revision Date: 07/31/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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