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Alexa Fluor® 700 anti-human CD27 Antibody

Pricing & Availability
Clone
O323 (See other available formats)
Regulatory Status
RUO
Workshop
IV T-186
Other Names
S152, T14, TNFRSF7
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
O323_Alx700_022008
Human peripheral blood lymphocytes stained with O323 Alexa Fluor® 700
  • O323_Alx700_022008
    Human peripheral blood lymphocytes stained with O323 Alexa Fluor® 700
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability Save
302813 25 µg 100€
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302814 100 µg 212€
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Description

CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70 and plays an important role in costimulation of T cell activation, and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application References
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations
  1. Sun C, et al. 2022. Nat Biomed Eng. 6:1004. PubMed
  2. Woodcock V, et al. 2023. BJUI Compass. 4:322. PubMed
  3. Ravens S, et al. 2020. Proc Natl Acad Sci U S A. 117:18649. PubMed
  4. Sauce D, et al. 2012. J Immunol. 189:5541. PubMed
  5. Esaulova E, et al. 2020. Cell Host Microbe. 29(2):165-178.e8. PubMed
  6. Eller MA, et al. 2016. J Virol. 90: 4005-4016. PubMed
  7. Fali T, et al. 2019. J Immunol. 202:1962. PubMed
  8. Onodera T, et al. 2019. J Immunol. 203:3282. PubMed
  9. Sayin I, et al. 2018. J Exp Med. 7:40286. PubMed
  10. Buggert M, et al. 2020. Cell. 183(7):1946-1961.e15. PubMed
  11. Wang X, et al. 2019. J Immunol. 203:2171. PubMed
  12. Rouers A, et al. 2021. iScience. 24:102482. PubMed
  13. Cherukuri A, et al. 2014. J Am Soc Nephrol. 7:1575. PubMed
  14. NULL, et al. 2022. Cell. 185:916. PubMed
  15. Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed
  16. Almeida J, et al. 2007. J Exp Med. 204:2473. PubMed
  17. Papagno L, et al. 2020. Sci Rep. 10:11620. PubMed
  18. Sauce D, et al. 2011. Blood. 117:5142. PubMed
RRID
AB_493756 (BioLegend Cat. No. 302813)
AB_493756 (BioLegend Cat. No. 302814)

Antigen Details

Structure
TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
Distribution

Medullary thymocytes, T and B cell subsets, NK cells

Function
T cell costimulation
Ligand/Receptor
CD70
Cell Type
B cells, NK cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Hintzen R, et al. 1994. Immunol. Today 15:307.
2. Agematsu K, et al. 1995. J. Immunol. 154:3627.

Gene ID
939 View all products for this Gene ID
UniProt
View information about CD27 on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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