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Alexa Fluor® 700 anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
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Product Citations
publications
RA3-6B2_Alx700_080408.jpg
C57BL/6 mouse splenocytes stained with RA3-6B2 Alexa Fluor® 700
  • RA3-6B2_Alx700_080408.jpg
    C57BL/6 mouse splenocytes stained with RA3-6B2 Alexa Fluor® 700
Compare all formats See Alexa Fluor® 700 spectral data
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103231 25 µg 76€
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103232 100 µg 169€
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Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD45R antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Application References
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  15. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Rydyznski CE et al. 2018. Cell reports. 24(13):3367-3373 . PubMed
  2. Schönberger K, et al. 2023. Anal Chem. 95:4325. PubMed
  3. Yamin R, et al. 2021. Nature. 599:465. PubMed
  4. MacLean AJ, et al. 2022. Immunity. 55:718. PubMed
  5. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  6. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  7. Feng J, et al. 2022. Immunity. 55:405. PubMed
  8. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  9. Groenen AG, et al. 2022. J Lipid Res. 63:100167. PubMed
  10. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  11. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  12. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  13. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  14. Liu Y, et al. 2022. Nat Commun. 13:2665. PubMed
  15. Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
  16. Shang Y, et al. 2016. Nat Immunol. 10.1038/ni.3486. PubMed
  17. Perry JSA, et al. 2018. Immunity. 48:923. PubMed
  18. Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed
  19. Onodera T, et al. 2019. J Immunol. 203:3282. PubMed
  20. Ruf B, et al. 2021. Cancer Immunol Res. 9:1024. PubMed
  21. Agrawal M, et al. 2021. Cells. 10:. PubMed
  22. Boyoglu-Barnum S, et al. 2014. J Virol. 88:10569. PubMed
  23. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
  24. Onodera T, et al. 2016. J Immunol. 196: 4172 - 4184. PubMed
  25. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  26. Peauroi EM, et al. 2022. iScience. 25:105510. PubMed
  27. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  28. La Rose AM, et al. 2021. Mol Metab. 53:101265. PubMed
  29. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  30. Spix B, et al. 2022. Nat Commun. 13:318. PubMed
  31. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  32. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  33. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  34. Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed
  35. Kumari P, et al. 2021. Cell Reports. 35(3):109012. PubMed
  36. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  37. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  38. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  39. Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
  40. Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
  41. Liu Q, et al. 2021. Adv Mater. 33:e2102852. PubMed
  42. Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
  43. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
  44. Adachi Y, et al. 2015. J Exp Med. 212: 1709-1723. PubMed
  45. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  46. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  47. Lee S, et al. 2021. Autophagy. 18:1062. PubMed
RRID
AB_493716 (BioLegend Cat. No. 103231)
AB_493716 (BioLegend Cat. No. 103232)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

Other Formats

View All CD45R/B220 Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F,FC,3D IHC
APC anti-mouse/human CD45R/B220 RA3-6B2 FC
Biotin anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
FITC anti-mouse/human CD45R/B220 RA3-6B2 FC
PE anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Cyanine5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
PE/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 488 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 421™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
Brilliant Violet 570™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 650™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 605™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 785™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 510™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,SB
Purified anti-mouse/human CD45R/B220 (Maxpar® Ready) RA3-6B2 FC,CyTOF®
Brilliant Violet 711™ anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Dazzle™ 594 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 750 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 750™ anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-A0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Spark Blue™ 550 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark NIR™ 685 anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-B0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Ultra-LEAF™ Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
TotalSeq™-C0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
PE/Fire™ 640 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Fire™ 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Violet™ 538 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 581 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 570 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F
PE/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Blue™ 574 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Violet™ 423 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Red™ 718 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark UV™ 387 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark PLUS UV395™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Go To Top Version: 3    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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