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Alexa Fluor® 647 anti-human CD20 Antibody

Pricing & Availability
Clone
C20Mab-60 (See other available formats)
Regulatory Status
RUO
Other Names
B1, Bp35
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A_C20Mab-60_A647_CD20_Antibody_072123
Human peripheral blood lymphocytes were stained with anti-human CD19 FITC and anti-human CD20 (clone C20Mab-60) Alexa Fluor® 647 (left), or mouse IgG2a, κ Alexa Fluor® 647 isotype control (right).
  • A_C20Mab-60_A647_CD20_Antibody_072123
    Human peripheral blood lymphocytes were stained with anti-human CD19 FITC and anti-human CD20 (clone C20Mab-60) Alexa Fluor® 647 (left), or mouse IgG2a, κ Alexa Fluor® 647 isotype control (right).
  • B_C20Mab-60_A647_Antibody_IHCP_082423
    IHC staining of anti-human CD20 (clone C20Mab-60) Alexa Fluor® 647 on paraffin-embedded human spleen tissue. Following antigen retrieval using Tris-EDTA, pH 9.0, the tissue was incubated with 5 µg/mL of anti-human CD8a Alexa Fluor® 594 (green) and 10 µg/mL of anti-human CD20 (clone C20Mab-60) Alexa Fluor® 647 (red) (panel A) or mouse IgG2a, κ Alexa Fluor® 647 isotype control (red) (panel B) at 4°C overnight. The nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective. Scale bar: 50 µm
Compare all formats See Alexa Fluor® 647 spectral data
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382803 25 µg 132€
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382804 100 µg 310€
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Description

CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
LN229/CD20 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
RRID
AB_3068232 (BioLegend Cat. No. 382803)
AB_3068232 (BioLegend Cat. No. 382804)

Antigen Details

Structure
Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
Distribution

B cell, T cell subsets

Function
B cell activation
Ligand/Receptor
Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Cell Type
B cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Furusawa Y, et al. 2020. Monoclon Antib Immunodiagn Immunother. 39:112-116.
Gene ID
931 View all products for this Gene ID
UniProt
View information about CD20 on UniProt.org
Go To Top Version: 1    Revision Date: 07/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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