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Anti-human CD3 FITC/(CD16+CD56) PE Cocktail

Pricing & Availability
Clone
UCHT1; 3G8; MEM-188;
Regulatory Status
RUO
Isotype
Mouse IgG1/Mouse IgG2a
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Product Citations
publications
CD16-CD56_PE_Cocktail_010521.png
Human peripheral blood mononuclear cells were stained with anti-human CD3 FITC/(CD16+CD56) PE cocktail. Dot plot is gated on lymphocyte population.
  • CD16-CD56_PE_Cocktail_010521.png
    Human peripheral blood mononuclear cells were stained with anti-human CD3 FITC/(CD16+CD56) PE cocktail. Dot plot is gated on lymphocyte population.
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319101 50 tests 184€
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Description

CD3: UCHT1 antibody reacts with a combinatorial epitope of CD3ε, a 20 kD chain of the CD3/T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells and some thymocytes.
CD16: 3G8 antibody reacts with CD16, a 50-65 kD transmembrane or a 48 kD GPI-anchored glycoprotein that is a member of the immunoglobulin superfamily. CD16 is expressed on NK cells, activated monocytes, macrophages, mast cells and neutrophils.
CD56: MEM-188 antibody reacts with the 175 - 185 kD isoform of CD56, N-CAM (Neural Cell Adhesion Molecule), which is expressed on NK cells and a subset of CD3+ T cells. CD56 is also expressed in brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
This reagent is a combination of FITC conjugated UCHT1 and PE conjugated 3G8 and MEM-188 in optimal concentration for two-color flow cytometric analysis.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Note:
Single color controls are required to perform instrument compensation and are not included in the kit. 

Product Citations
  1. Jeff Baker, Joshua Nederveen, Gianni Parise 2017. J Appl Physiol. 122(1):182-190. PubMed
RRID
AB_314999 (BioLegend Cat. No. 319101)

Antigen Details

Distribution

T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells and some thymocytes

Cell Type
Mesenchymal Stem Cells, NKT cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.
4. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275.
5. Stroncek D, et al. 1991. Blood 77:1572.
6. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.
7. Lanier L, et al. 1991. J. Immunol. 146:4421.
8. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71.
9. Cremer H, et al. 1994. Nature 367:455.

Gene ID
920 View all products for this Gene ID

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

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Go To Top Version: 4    Revision Date: 01/05/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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