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APC anti-mouse/human CD15 (SSEA-1) Antibody

Pricing & Availability
Clone
MC-480 (See other available formats)
Regulatory Status
RUO
Other Names
Stage-Specific Embryonic Antigen 1, X-hapten, Lewis X, 3-FAL, CD15, SSEA1, SSEA-1
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
MC-480_APC_CD15_Antibody_021419
Murine embryonic carcinoma cell line F9 was stained with CD15 (SSEA-1) (clone MC-480) APC (filled histogram) or mouse IgM APC Isotype control (open histogram).
  • MC-480_APC_CD15_Antibody_021419
    Murine embryonic carcinoma cell line F9 was stained with CD15 (SSEA-1) (clone MC-480) APC (filled histogram) or mouse IgM APC Isotype control (open histogram).
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125617 25 tests 120€
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125618 100 tests 252€
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Description

The MC-480 antibody reacts with mouse and human Stage-Specific Embryonic Antigen-1 (SSEA-1). It is a Lewis blood group related carbohydrate antigen, also known as X-hapten, Lewis X, 3-FAL, 3-fucosyl-N-acetyllactosamine, or CD15. The expression pattern of SSEA-1 antigen is different in humans than in mice. In mice, SSEA-1 is expressed on embryonic stem cells (ES), embryonal carcinoma cells (EC), 8-cell to blastocyst embryos, and a subset of embryonic inner cell mass. The expression on murine ES cells is decreased upon differentiation. In humans, however, SSEA-1 is not found on undifferentiated ES cells, but its expression is upregulated along with differentiation. CD15 is highly expressed on adult human granulocytes. It has been reported that SSEA-1 plays a role in cell adhesion and regulation of cell differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse F9 Teratocarcinoma Stem Cells (X-irradiated)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.

Application References
  1. Solter D and Knowles BB. 1978. Proc. Natl. Acad. Sci. USA. 75:5565. (IHC, IP, WB)
Product Citations
  1. Inoue S, et al. 2014. Crit Care. R130:18. PubMed
  2. Bartoccetti M, et al. 2020. Cell Reports. 30(7):2150-2169. PubMed
RRID
AB_2800605 (BioLegend Cat. No. 125617)
AB_2800605 (BioLegend Cat. No. 125618)

Antigen Details

Structure
Carbohydrate epitope, Lewis blood group antigens
Distribution

Murine embryonic stem cells (ES), embryonal carcinoma cells, 8-cell to blastocyst embryos; human differentiated cells, not on undifferentiated ES cells

Function
Adhesion, differentiation
Cell Type
Embryonic Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Solter D and Knowles BB. 1978. Proc. Natl. Acad. Sci. USA. 75:5565.
2. Harris JF, et al. 1984. J. Immunol. 132:2502.
3. Gooi HC, et al. 1981. Nature 292:156.
4. Cui L, et al. 2004. J. Histochem. Cytochem. 52:1447.

Gene ID
14345 View all products for this Gene ID 2526 View all products for this Gene ID
UniProt
View information about CD15 on UniProt.org
Go To Top Version: 1    Revision Date: 02/14/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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