Biotin anti-human CD196 (CCR6) Antibody, CD196, G034E3

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Clone: G034E3 (See other available formats)
Regulatory Status: RUO
Other Names: BN-1, CKR6, DCR2, CKRL3, DRY-6, GPR29, CKR-L3, CMKBR6, GPRCY4, STRL22, GPR-CY4
Isotype: Mouse IgG2b, κ
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G034E3_Biotin_CD196_Antibody_1_011024 — Human peripheral lymphocytes were stained with anti-human CD19 APC and biotinylated anti-human CD196 (CCR6) (clone G034E3) (left) or biotinylated mouse IgG2b, κ isotype control (right), followed by Sav-PE.

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353462

100 µg

100€

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Description

CCR6, also known as CD196, is a chemokine receptor that is expressed on immature dendritic cells, B lymphocytes, and memory T cells. CCR6 binds CCL20, although members of the β defensin family also bind CCR6 with a lower affinity. CCR6 positive cells, and its ligand CCL20, have been detected in numerous organs, especially the secondary lymphoid organ. CCL20 is selectively made by the follicle-associated epithelium (FAE) overlying Peyers Patches (PPs) and isolated lymphoid follicles (ILFs). CCL20 contributes to the recruitment of CCR6-expressing B cells to these structures. In humans, CCR6 can function to mediate arrest of T cells on dermal endothelial cells and is highly expressed on T cells resident in both normal and psoriatic skin. CCR6 and/or CCL20 have been implicated in the pathogenesis of rheumatoid arthritis and inflammatory bowel disease. Human T cells that are able to produce IL-17 express CCR6. It suggests that CCL20 and CCR6 have a role in inflammatory diseases by recruiting Th17 cells to target tissues.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CCR6-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3106015 (BioLegend Cat. No. 353462)

Antigen Details

Structure: Chemokine receptor, GPCR, seven transmembrane receptor
Distribution: Immature dendritic cells, B lymphocytes, memory T cells, Th17 cells
Function: Host defense, important for recruitment of B cells to secondary lymphoid organs, mediates arrest of T cells on dermal endothelial cells
Ligand/Receptor: CCL20 (MIP-3α)
Cell Type: B cells, Dendritic cells, T cells, Th17
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Zaballos A, et al. 1996. Biochem. Bioph. Res. Co. 227:846.
2. Yang D, et al. 1999. Science 286:525.
3. MacDonald KG, et al. 2007. Am. J. Pathol. 170:1229.
4. Homey B, et al. 2000. J. Immunol. 164:6621.
5. Hirota K, et al. 2007. J. Exp. Med. 204:2803.
6. Singh SP, et al. 2008. J. Immunol. 180:214.
Gene ID: 1235 View all products for this Gene ID
UniProt: View information about CD196 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Other Formats

View All CD196 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD196 (CCR6)	G034E3	FC
Alexa Fluor® 647 anti-human CD196 (CCR6)	G034E3	FC
Brilliant Violet 421™ anti-human CD196 (CCR6)	G034E3	FC
PerCP/Cyanine5.5 anti-human CD196 (CCR6)	G034E3	FC
PE anti-human CD196 (CCR6)	G034E3	FC
FITC anti-human CD196 (CCR6)	G034E3	FC
Alexa Fluor® 488 anti-human CD196 (CCR6)	G034E3	FC
APC anti-human CD196 (CCR6)	G034E3	FC
PE/Cyanine7 anti-human CD196 (CCR6)	G034E3	FC
Brilliant Violet 605™ anti-human CD196 (CCR6)	G034E3	FC
Brilliant Violet 785™ anti-human CD196 (CCR6)	G034E3	FC
Brilliant Violet 510™ anti-human CD196 (CCR6)	G034E3	FC
Brilliant Violet 650™ anti-human CD196 (CCR6)	G034E3	FC
Purified anti-human CD196 (CCR6) (Maxpar® Ready)	G034E3	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD196 (CCR6)	G034E3	FC
APC/Cyanine7 anti-human CD196 (CCR6)	G034E3	FC
Alexa Fluor® 700 anti-human CD196 (CCR6)	G034E3	FC
Brilliant Violet 711™ anti-human CD196 (CCR6)	G034E3	FC
TotalSeq™-A0143 anti-human CD196 (CCR6)	G034E3	PG
Pacific Blue™ anti-human CD196 (CCR6)	G034E3	FC
TotalSeq™-C0143 anti-human CD196 (CCR6)	G034E3	PG
APC/Fire™ 750 anti-human CD196 (CCR6)	G034E3	FC
TotalSeq™-B0143 anti-human CD196 (CCR6)	G034E3	PG
TotalSeq™-D0143 anti-human CD196 (CCR6)	G034E3	PG
PE/Fire™ 640 anti-human CD196 (CCR6)	G034E3	FC
APC/Fire™ 810 anti-human CD196 (CCR6)	G034E3	FC
Spark NIR™ 685 anti-human CD196 (CCR6)	G034E3	FC
PE/Fire™ 700 anti-human CD196 (CCR6)	G034E3	FC
PE/Cyanine5 anti-human CD196 (CCR6)	G034E3	FC
Biotin anti-human CD196 (CCR6)	G034E3	FC
PE/Fire™ 810 anti-human CD196 (CCR6)	G034E3	FC
PerCP/Fire™ 780 anti-human CD196 (CCR6) Antibody	G034E3	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 01/10/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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