Biotinylated Recombinant Human CD40 (TNFRSF5)-Fc Chimera (carrier-free)

Product Details

Source

Human CD40 amino acid Glu21-Arg193 (Accession: #P25942.1), with a linker (GS), a C-terminal human IgG1 (Pro100-Lys330) and an Avi-tag, was expressed in 293E cells. Human CD40-Fc Chimera was site-specifically biotinylated by enzyme BirA.

Molecular Mass

The 421 amino acid recombinant protein has a predicted molecular mass of approximately 47.06 kD. The DTT-reduced and non-reduced protein migrates at approximately 62 kD and 110 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Glu.

Purity

> 95%, as determined by Coomassie stained SDS-PAGE

Formulation

0.22 µm filtered protein solution is in PBS, 5% glycerol.

Endotoxin Level

Less than 0.1 EU per µg protein as determined by the LAL method.

Concentration

10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Activity

When recombinant human CD40L (Cat. No. 591702) is immobilized at 1 μg/mL, biotinylated recombinant human CD40-Fc chimera binds in a dose-dependent manner with EC₅₀ range of 50-200 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.

Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure

Disulfide linked homodimer

Distribution

B cells, macrophages, monocytes, and dendritic cells

Function

CD40 regulates B-cell development/maturation, Ig isotype switching proliferation. CD40 is also involved in T-cell priming and inflammatory cytokine production.

Interaction

TRAF1, 2, 3, 5, 6

Ligand/Receptor

TNFSF5/CD40L

Bioactivity

Measured by its ability to bind recombinant human CD40L

Antigen References

1. Ridge JP, et al. 1998. Nature 393:474.
2. Schoenberger SP, et al. 1998. Nature 393:480.
3. Bremer E. 2013. ISRN Oncol. 2013:371854.
4. Siddiqa A, et al. 2001. Nature 410: 383.
5. Durandy A, et al. 2005. Immunol. Rev. 203:67.
6. O'Grady JT, et al. 1994. Am. J. Pathol. 144:21.
7. Karaca NE, et al. 2012. Scand. J. Immunol. 76:21.
8. Tone M, et al. 2001. Proc. Natl. Acad. Sci. USA. 98:1751.
9. Eshel D, et al. 2008. Mol. Immunol. 46:250.
10. Hostager BS, et al. 2000. J. Biol. Chem. 275:15392.
11. Elgueta R, et al. 2009. Immunol. Rev. 229:152.

Gene ID

958 View all products for this Gene ID

UniProt

View information about CD40 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

Certificate of Analysis