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Brilliant Violet 421™ anti-human CD125 (IL-5Rα) Antibody

Pricing & Availability
Clone
S20015E (See other available formats)
Regulatory Status
RUO
Other Names
IL5RA, IL5R, CDw125, HSIL5R3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
S20015E_BV421_CD125_FC_031225-4
Human peripheral blood lymphocytes were stained with anti-human CD193 (CCR3) (clone 5E8) FITC and anti-human CD125 (IL-5Rα) (clone S20015E) Brilliant Violet 421™ (left) or mouse IgG1, κ Brilliant Violet 421™ isotype control (right).
  • S20015E_BV421_CD125_FC_031225-4
    Human peripheral blood lymphocytes were stained with anti-human CD193 (CCR3) (clone 5E8) FITC and anti-human CD125 (IL-5Rα) (clone S20015E) Brilliant Violet 421™ (left) or mouse IgG1, κ Brilliant Violet 421™ isotype control (right).
Compare all formats See Brilliant Violet 421™ spectral data
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384711 25 tests 155€
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384712 100 tests 326€
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Description

CD125, also known as IL5 receptor α-chain, is a 60 kD type I transmembrane protein that binds IL-5 and associates in a heterodimer with the signal-transducing β subunit CD131 to form the IL-5 receptor. The affinity of CD125 for IL-5 is increased when is associated with CD131. The cytoplasmic region of CD125 activates the JAK2/STAT5 pathway and the kinase Btk. CD125 is expressed by eosinophils, basophils, mast cells, B1 cells and a subset of resting B2 cells and have a central role in the differentiation, activation and survival of these cells. Due to the restricted expression of this molecule, it is considered a potential therapeutic target for allergic inflammatory conditions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse IL-5Rα transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)

Antigen Details

Structure
Type I transmembrane protein, 60 kD
Distribution

Eosinophils, basophils, mast cells, B1 cells, subset of resting B2 cells

Function
Role in eosinophils and basophils differentiation, activation and survival
Interaction
JAK2/STAT, Btk, SOX4
Ligand/Receptor
IL-5
Cell Type
B cells, Basophils, Eosinophils
Biology Area
Cell Biology, Cell Proliferation and Viability, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Buchheit KM, et al. 2020. J Allergy Clin Immunol. 145:1574-1584.
  2. Lin X, et al. 2020. Inflammation. 43:1797-1805.
  3. Gorski SA, et al. 2019. PLoS One. 14:e0221113.
  4. Varricchi G, et al. 2016. Curr Opin Allergy Clin Immunol.16:186-200.
  5. Beekman JM. et al. 2009. Blood. 114:3917-27.
  6. Lei JT. et al. 2008. J Leukoc Biol. 84:499-509.
Gene ID
3568 View all products for this Gene ID
UniProt
View information about CD125 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 03/12/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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