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Brilliant Violet 421™ anti-human CD14 Antibody

Pricing & Availability
Clone
HCD14 (See other available formats)
Regulatory Status
RUO
Other Names
LPS receptor
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HCD14_BV421_040711
Human peripheral blood monocytes were stained with CD14 (clone HCD14) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • HCD14_BV421_040711
    Human peripheral blood monocytes were stained with CD14 (clone HCD14) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
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325627 25 tests 159€
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325628 100 tests 322€
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Application References
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. Cirovic B, et al. 2020. Cell Host & Microbe. 28(2):322-334. PubMed
  2. Sulaj A, et al. 2022. J Clin Endocrinol Metab. 107:2167. PubMed
  3. Pioch J, et al. 2022. J Immunol Methods. 507:113308. PubMed
  4. Varun K, et al. 2023. EBioMedicine. 90:104516. PubMed
  5. Toriyama M, et al. 2023. Front Mol Biosci. 10:1149828. PubMed
  6. Qiu J, et al. 2020. Cell Rep. 33:108465. PubMed
  7. Ibidapo-Obe O, et al. 2020. Cell Mol Gastroenterol Hepatol. 9:661. PubMed
  8. Walsh RE, et al. 2020. MAbs. 12:1764829. PubMed
  9. Forconi CS, et al. 2020. Frontiers in Cellular and Infection Microbiology. 0.529166667. PubMed
  10. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  11. Watson D, et al. 2016. Biomaterials. 105:195-205. PubMed
  12. Richert-Spuhler LE, et al. 2021. Cell Reports Medicine. 2(6):100322. PubMed
  13. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  14. Jost M, et al. 2021. eLife. 10:00. PubMed
  15. Zhichao Fan et al. 2019. Cell reports. 26(1):119-130 . PubMed
  16. Feng S, et al. 2022. Front Immunol. 13:990522. PubMed
  17. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
  18. Dos Santos JC, et al. 2019. Cell Rep. 28:2659. PubMed
  19. Forsberg MH, et al. 2021. Stem Cell Res Ther. 12:459. PubMed
  20. Frei A, et al. 2016. Nat Methods. 10.1038/nmeth.3742. PubMed
  21. Joly P, et al. 2017. PLoS One. 12(6):e0180568. PubMed
  22. Fan Z, et al. 2020. STAR Protoc. 1:100012. PubMed
  23. Hegewisch-Solloa E, et al. 2021. J Immunol. 207:950. PubMed
  24. Burel JG, et al. 2019. Elife. 8. PubMed
  25. Hilt ZT, et al. 2019. JCI Insight. 4:5. PubMed
  26. Gamradt S, et al. 2021. iScience. 24:103312. PubMed
RRID
AB_2561342 (BioLegend Cat. No. 325627)
AB_2561342 (BioLegend Cat. No. 325628)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 07/13/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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