- Clone
- 16B5C71 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IK1, IKZF1
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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368405 | 25 tests | 155€ | ||||
368406 | 100 tests | 348€ |
Ikaros, also known as IKZF1, belongs to a family of zinc-finger DNA-binding proteins that regulate lymphocyte differentiation. It has the ability to form homo- and heterodimers with other members of the Ikaros family, which include Aiolos and Helios. Ikaros can be found on a majority of fetal and adult hematopoietic cell types such as T- and B-cells, natural killer cells (NKs), monocytes/macrophages, and dendritic cells. Ikaros is a tumor suppressor and its absence or reduced expression has been reported in several leukemias.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Ikaros peptide (431-477 aa) conjugated to KLH Freund's adjuvant.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
There are eight splicing variants with predicted MW 58, 53, 48(2), 43, 41, 31, 24 KDa. This antibody might recognize some of the isoforms.
- RRID
-
AB_2571956 (BioLegend Cat. No. 368405)
AB_2571956 (BioLegend Cat. No. 368406)
Antigen Details
- Structure
- Zinc-finger DNA binding protein, homo/heterodimer, 57.5 kD
- Distribution
-
Fetal and adult hematopoietic cells.
- Function
- Regulates lymphocyte differentiation.
- Interaction
- DNA.
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Wargnier A, et al. 1995. Proc. Natl. Acad. Sci. USA 92:6930.
2. Tonnelle C, et al. 2009. Mol. Immunol. 46:1736.
3. Wang H, et al. 2014. Pediatr. Blood Cancer 61:2230. - Gene ID
- 10320 View all products for this Gene ID
- UniProt
- View information about Ikaros on UniProt.org
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
-
It is lot-specific. On average it ranges between 2-4.
Other Formats
View All Ikaros Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human Ikaros | 16B5C71 | WB,ChIP |
Alexa Fluor® 647 anti-human Ikaros | 16B5C71 | ICFC |
Brilliant Violet 421™ anti-human Ikaros | 16B5C71 | ICFC |
Alexa Fluor® 488 anti-human Ikaros | 16B5C71 | ICFC |
PE anti-human Ikaros | 16B5C71 | ICFC |
APC anti-human Ikaros | 16B5C71 | ICFC |
PE/Cyanine7 anti-human Ikaros | 16B5C71 | ICFC |
PerCP/Cyanine5.5 anti-human Ikaros | 16B5C71 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human Ikaros
Total lysates (15 µg protein) from Jurkat (lane 1) and HeLa ... Chromatin Immunoprecipitations (ChIP) were done with fixed a... Chromatin Immunoprecipitations (ChIP) were done with fixed a... -
Alexa Fluor® 647 anti-human Ikaros
Human peripheral blood mononuclear cells were stained with C... -
Brilliant Violet 421™ anti-human Ikaros
Human peripheral blood mononuclear cells were stained with C... -
Alexa Fluor® 488 anti-human Ikaros
Human peripheral blood mononuclear cells were stained with C... -
PE anti-human Ikaros
Human peripheral blood mononuclear cells were stained with C... -
APC anti-human Ikaros
Human peripheral blood mononuclear cells were stained with C... -
PE/Cyanine7 anti-human Ikaros
Human peripheral blood mononuclear cells were stained with C... -
PerCP/Cyanine5.5 anti-human Ikaros
Human peripheral blood mononuclear cells were surface staine...
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