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Brilliant Violet 421™ anti-mouse ENPP1 Antibody

Pricing & Availability
Clone
YE1/19.1 (See other available formats)
Regulatory Status
RUO
Other Names
Ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), Ly-41, PC-1, NPP1, Plasma Cell Alloantigen 1 (PC1)
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
YE1slash19.1_BV421_ENPP1_Antibody_FC_031815
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC, CD138 PE, and ENPP1 (clone YE1/19.1) ™ (filled histogram) or rat IgG2b, κ Brilliant Violet 421™ isotype control (open histogram). Data shown was gated on the plasma cell (B220- CD138+) population.
  • YE1slash19.1_BV421_ENPP1_Antibody_FC_031815
    C57BL/6 mouse splenocytes were stained with CD45R/B220 APC, CD138 PE, and ENPP1 (clone YE1/19.1) ™ (filled histogram) or rat IgG2b, κ Brilliant Violet 421™ isotype control (open histogram). Data shown was gated on the plasma cell (B220- CD138+) population.
Compare all formats See Brilliant Violet 421™ spectral data
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149207 50 µg 208€
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Description

ENPP1, also known as PC-1 or Ly-41, is a 115 kD type II transmembrane enzyme that is located on the cell's surface as a homodimer. ENPP1 hydrolyses ATP and other nucleoside 5' triphosphates to produce pyrophosphate which is an inhibitor of calcification and bone formation. It is involved in nucleotide recycling, cell adhesion, and regulates the signaling of insulin and purinergic receptors. ENPP1 is expressed by plasma cells, subsets of T and B1 cells, chondrocytes, capillary endothelium of the brain, renal distal tubules, and hepatocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine T cell lymphoma EL4.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

This clone recognizes both alleles of ENPP1.

Additional reported applications (for the relevant formats) include: Immunoprecipitation.2

Application References
  1. Wang H, et al. 2012. Proc Natl Acad Sci USA 109:20077. (FC)
  2. Abbasi S, et al. 2011. Hybridoma 30:11. (FC, IP)
  3. Waterfield JD and Fairhurst M. 1991. Immunology 72:226. (FC)
  4. Takei F. 1984. J. Immunol. 4:1951. (FC, IP)
Product Citations
  1. Wolock SL, et al. 2019. Cell Rep. 28:302. PubMed
RRID
AB_2565483 (BioLegend Cat. No. 149207)

Antigen Details

Structure
Type II transmembrane protein, 115 kD, and forms homodimers.
Distribution
Plasma cells, a subset of T cells, a subset of B1 cells, chondrocytes, capillary endothelium of the brain, renal distal tubules, and hepatocytes.
Function
Generate pyrophosphate, recycle nucleotides, regulate cell adhesion, and to regulate signaling of insulin and purinergenic receptors.
Cell Type
Plasma cells, T cells, B cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
Phospho-Proteins
Antigen References

1. Lau WM, et al. 2013. PLoS One. 8:e66752.
2. Kato K, et al. 2012. Proc. Natl. Acad. Sci. USA 109:16876.
3. Nam HK, et al. 2011. J Biol. Chem. 286:39059.
4. Zhou HH, et al. 2009. Eur. J. Pharmacol. 616:346.

Gene ID
18605 View all products for this Gene ID
UniProt
View information about ENPP1 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 03/18/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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