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Brilliant Violet 510™ anti-human CD152 (CTLA-4) Antibody

Pricing & Availability
Clone
BNI3 (See other available formats)
Regulatory Status
RUO
Other Names
CTLA4, Cytotoxic T-Lymphocyte Antigen 4, CTLA-4
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A. BNI3_BV-510_CD152_1_FC_072224
Human peripheral blood mononuclear cells were stimulated with Cell Activation Cocktail (without Brefeldin A) (Cat No. 423302) for 4 hours, stained with anti-human CD3 (clone UCHT1) PerCP/Cyanine5.5, fixed using Fixation Buffer (Cat. No. 420801) and permeabilized using Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002). Cells were then intracellularly stained with anti-human CD152 (CTLA-4) (clone BNI3) Brilliant Violet 510™ (left), or mouse IgG2a, κ Brilliant Violet 510™ isotype control (right).
  • A. BNI3_BV-510_CD152_1_FC_072224
    Human peripheral blood mononuclear cells were stimulated with Cell Activation Cocktail (without Brefeldin A) (Cat No. 423302) for 4 hours, stained with anti-human CD3 (clone UCHT1) PerCP/Cyanine5.5, fixed using Fixation Buffer (Cat. No. 420801) and permeabilized using Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002). Cells were then intracellularly stained with anti-human CD152 (CTLA-4) (clone BNI3) Brilliant Violet 510™ (left), or mouse IgG2a, κ Brilliant Violet 510™ isotype control (right).
  • B. BNI3_BV-510_CD152_2_FC_072224
    PHA-stimulated human peripheral blood mononuclear cells (3 days) were stained with anti-human CD3 (clone UCHT1) APC and anti-human CD152 (CTLA-4) (clone BNI3) Brilliant Violet 510™ (left), or mouse IgG2a, κ Brilliant Violet 510™ isotype control (right).
Compare all formats See Brilliant Violet 510™ spectral data
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369649 25 tests 155€
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369650 100 tests 326€
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Description

CD152, also known as Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), is a 33 kD member of the immunoglobulin superfamily. It is transiently expressed on activated T cells. CTLA-4 is expressed on the surface of helper T cells and transmits an inhibitory signal to T cells. Regulatory T cells express high levels of CTLA-4. CTLA-4 (CD152) is similar to CD28 in amino acid sequence, structure, and genomic organization. Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses through interaction with CD80 (B7-1) and CD86 (B7-2) present on antigen presenting cells (APC). CTLA-4 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.

Mutations in the CTLA-4 gene have been associated with various autoimmune diseases, such as systemic lupus erythematosus, insulin-dependent diabetes mellitus, and other autoimmune diseases. A transcript of the CTLA-4 gene that may represent a native soluble form of CTLA-4 (sCTLA-4) showed that eleven of twenty patients with autoimmune thyroid disease (ATD) had a high concentration of sCTLA-4, whereas only 1 of 30 apparently healthy volunteers contained measurable levels. sCTLA-4 immunoreactivity was inhibited by its binding to B7.1, suggesting that sCTLA-4 is a functional receptor. sCTLA-4 also plays a role in the initial immune response to infection of immune cells by HIV, along with the CD-1 pathway and others.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Extracellular domain of human CTLA-4 and constant regions of the human IgG heavy chain (CTLA-4/IgG)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Based on in-house testing, we do not recommend using clone BNI3 for immunohistochemistry of paraffin-embedded tissue section.

Application References
  1. Linsley PS, et al. 1992. J. Exp. Med. 176:1595.
  2. Bonzheim I, et al. 2008. Am. J. Clin. Pathol. 130:613.
RRID
AB_3662273 (BioLegend Cat. No. 369649)
AB_3662273 (BioLegend Cat. No. 369650)

Antigen Details

Structure
Ig superfamily and 33 kD.
Distribution

Activated T cells and B cells.

Function
Negative regulator of T cell activation.
Interaction
Antigen presenting cells, such as dendritic cells.
Ligand/Receptor
B7-1 (CD80), B7-2 (CD86).
Cell Type
B cells, T cells
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Kuiper HM, et al. 1995. J. Immunol. 155:1776.
2. Castan J, et al. 1997. Immunology 90:265.
3. Lee CC, et al. 2009. Pediatr. Allergy Immunol. 20:624.
4. Pistillo MP, et al. 2003. Blood 101:202.
5. Tan PH, et al. 2005. Blood. 106:2936.
6. Steiner K, et al. 2001. Clin. Exp. Immunol. 126:143.

Gene ID
1493 View all products for this Gene ID
UniProt
View information about CD152 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD152 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD152 (CTLA-4) BNI3 ICFC,FC
PE anti-human CD152 (CTLA-4) BNI3 ICFC,FC
Brilliant Violet 421™ anti-human CD152 (CTLA-4) BNI3 ICFC,FC
Brilliant Violet 605™ anti-human CD152 (CTLA-4) BNI3 ICFC,FC
PerCP/Cyanine5.5 anti-human CD152 (CTLA-4) BNI3 ICFC,FC
Biotin anti-human CD152 (CTLA-4) BNI3 ICFC,FC
APC anti-human CD152 (CTLA-4) BNI3 ICFC,FC
PE/Cyanine7 anti-human CD152 (CTLA-4) BNI3 ICFC,FC
PE/Dazzle™ 594 anti-human CD152 (CTLA-4) BNI3 ICFC,FC
TotalSeq™-A0151 anti-human CD152 (CTLA-4) BNI3 PG
TotalSeq™-C0151 anti-human CD152 (CTLA-4) BNI3 PG
Brilliant Violet 785™ anti-human CD152 (CTLA-4) BNI3 ICFC,FC
TotalSeq™-B0151 anti-human CD152 (CTLA-4) BNI3 PG
APC/Fire™ 750 anti-human CD152 (CTLA-4) BNI3 ICFC,FC
Alexa Fluor® 647 anti-human CD152 (CTLA-4) BNI3 ICFC,FC
APC/Cyanine7 anti-human CD152 (CTLA-4) Antibody BNI3 ICFC,FC
Brilliant Violet™ 711 anti-human CD152 (CTLA-4) BNI3 ICFC,FC
TotalSeq™-D0151 anti-human CD152 (CTLA-4) BNI3 PG
PE/Fire™ 640 anti-human CD152 (CTLA-4) BNI3 ICFC,FC
Spark Red™ 718 anti-human CD152 (CTLA-4) BNI3 ICFC,FC
PE/Cyanine5 anti-human CD152 (CTLA-4) BNI3 ICFC,FC
PE/Fire™ 744 anti-human CD152 (CTLA-4) BNI3 ICFC,FC
PE/Fire™ 810 anti-human CD152 (CTLA-4) BNI3 ICFC,FC
Brilliant Violet 650™ anti-human CD152 (CTLA-4) BNI3 ICFC,FC
Brilliant Violet 510™ anti-human CD152 (CTLA-4) BNI3 ICFC,FC
Spark Blue™ 574 anti-human CD152 (CTLA-4) (Flexi-Fluor™) BNI3 FC
Spark Blue™ 550 anti-human CD152 (CTLA-4) (Flexi-Fluor™) BNI3 FC
Go To Top Version: 1    Revision Date: 03/15/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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