- Clone
- I3/2.3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T200, Ly-5, LCA
- Isotype
- Rat IgG2b, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
147721 | 50 µg | 184€ |
CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from alternative splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse lymphoma cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin embedded sections1 and frozen tissue sections2.
- Application References
-
- Kliment C, et al. 2009. J. Mol. Cell Cardiol. 47:730. (IHC)
- Reynolds JM, et al. 2007. J. Immunol. 179:313. (IHC)
- RRID
-
AB_3662407 (BioLegend Cat. No. 147721)
Antigen Details
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
-
All hematopoietic cells except mature erythrocytes and platelets
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
- Cell Type
- B cells, Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules, TCRs
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS and Thomas ML. 1994. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851. - Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45 on UniProt.org
Other Formats
View All CD45 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD45 | I3/2.3 | FC,IHC-P,IHC-F,SB |
PerCP/Cyanine5.5 anti-mouse CD45 | I3/2.3 | FC |
PE/Cyanine7 anti-mouse CD45 | I3/2.3 | FC |
APC anti-mouse CD45 | I3/2.3 | FC |
FITC anti-mouse CD45 | I3/2.3 | FC |
PE anti-mouse CD45 | I3/2.3 | FC |
APC/Fire™ 750 anti-mouse CD45 | I3/2.3 | FC |
Alexa Fluor® 700 anti-mouse CD45 | I3/2.3 | FC |
APC/Cyanine7 anti-mouse CD45 Antibody | I3/2.3 | FC |
Brilliant Violet 421™ anti-mouse CD45 | I3/2.3 | FC |
Brilliant Violet 510™ anti-mouse CD45 | I3/2.3 | FC |
Brilliant Violet 605™ anti-mouse CD45 | I3/2.3 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-mouse CD45
C57BL/6 splenocytes were stained with purified CD45 (clone I... -
PerCP/Cyanine5.5 anti-mouse CD45
C57BL/6 splenocytes were stained with CD45 (clone I3/2.3) Pe... -
PE/Cyanine7 anti-mouse CD45
C57BL/6 splenocytes were stained with CD45 (clone I3/2.3) PE... -
APC anti-mouse CD45
C57BL/6 splenocytes were stained with CD45 (clone I3/2.3) AP... -
FITC anti-mouse CD45
C57BL/6 splenocytes were stained with CD45 (clone I3/2.3) FI... -
PE anti-mouse CD45
C57BL/6 splenocytes were stained with CD45 (clone I3/2.3) PE... -
APC/Fire™ 750 anti-mouse CD45
C57BL/6 mouse splenocytes were stained with CD45 (clone I3/2... -
Alexa Fluor® 700 anti-mouse CD45
C57BL/6 mouse splenocytes were stained with CD45 (clone I3/2... -
APC/Cyanine7 anti-mouse CD45 Antibody
C57BL/6 splenocytes were stained with anti-mouse CD45 (clone... -
Brilliant Violet 421™ anti-mouse CD45
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 (... C57BL/6 mouse splenocytes were stained with anti-mouse CD45 ... -
Brilliant Violet 510™ anti-mouse CD45
C57BL/6 mouse splenocytes were stained with anti-mouse CD45 ... -
Brilliant Violet 605™ anti-mouse CD45
C57BL/6 mouse splenocytes were stained with anti-mouse CD45 ...
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