Cell-Vive™ GMP Recombinant Human IL-10 (carrier-free)

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Other Names
Interleukin-10, B-TCGF, CSIF, TGIF
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GMP recombinant human IL-10 inhibits IFN-γ induction in PHA activated human PBMC in a dose-dependent manner with ED50 range of 0.025 - 0.25 ng/mL.
  • Human_IL-10_CF_GMP-RECOM_071321.png
    GMP recombinant human IL-10 inhibits IFN-γ induction in PHA activated human PBMC in a dose-dependent manner with ED50 range of 0.025 - 0.25 ng/mL.
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571014 25 µg 446€
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571016 100 µg 1500€
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

IL-10 was first described as a cytokine that is produced by T helper 2 (Th2) cell clones. It inhibits interferon (IFN)-γ synthesis in Th1 cells, and therefore it was initially called cytokine synthesis inhibiting factor (CSIF).  Macrophages are the main source of IL-10 and its secretion can be stimulated by endotoxin (via Toll-like receptor 4, NF-κB dependent), tumor necrosis factor TNF-α (via TNF receptor p55, NF-κB-dependent), catecholamines, and IL-1. IL-10 controls inflammatory processes by suppressing the expression of proinflammatory cytokines, chemokines, adhesion molecules, as well as antigen-presenting and costimulatory molecules in monocytes/macrophages, neutrophils, and T cells. IL-10 inhibits the production of proinflammatory mediators by monocytes and macrophages such as endotoxin- and IFN-γ-induced release of IL-1α, IL-6, IL-8, G-CSF, GM-CSF, and TNF-α. In addition, it enhances the production of anti-inflammatory mediators such as IL-1RA and soluble TNFα receptors. IL-10 inhibits the capacity of monocytes and macrophages to present antigen to T cells. This is realized by down-regulation of constitutive and IFN-γ-induced cell surface levels of MHC class II, of costimulatory molecules such as CD86 and of some adhesion molecules such as CD58.

Product Details
Quality Statement

BioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:

  • Low endotoxin level (≤ 0.1 EU/μg)
  • Purity (≥ 95% or higher)
  • Bioburden testing
  • Mycoplasma testing
  • Batch-to-batch consistency
  • Vendor qualification
  • Raw material traceability and documentation
  • Documented procedures and employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products
Technical Data Sheet (pdf)

Product Details

Source
Human IL-10, amino acids Ser19-Asn178 (Accession # NM_000572), was expressed in E.coli.
Molecular Mass
The 160 amino acid recombinant protein has a predicted molecular mass of 18 kD. The DTT-reduced protein migrates at approximately 18 kD and the non-reduced protein migrates at approximately 15 kD by SDS-PAGE. The N-terminal amino acid is Serine.
N-terminal
Sequence Analysis
Ser-Pro-Gly-Gln-Gly-Thr-Gln-Ser-Glu-Asn
Purity
≥ 95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.1 µm filtered protein solution is in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2.
Endotoxin Level
Less than or equal to 0.1 EU per µg protein as determined by the LAL method
Residual Host Cell Protein Content
≤ 0.500 ng/μg by ELISA
Concentration
500 µg/mL
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 0.025 - 0.25 ng/mL as determined by the dose-dependent inhibition of IFN-γ induction in PHA activated human PBMC. The specific activity of Cell-Vive™ GMP Recombinant Human IL-10 (carrier-free) is >1.3 x 106 IU/mg when compared against the WHO International Standard for Human IL-10 (NIBSC code: 93/722).
Application

Bioassay
Cell Culture

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Disclaimer

BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Antigen Details

Structure
Cytokine
Distribution

IL-10 is produce by Th2 cells, macrophages, DCs, B cells, CD8+ T cells, regulatory T cells (Tregs), Th1 cells and Th17 cells. In addition, IL-10 is expressed by monocytes, B cells, eosinophils, and mast cells.

Function
IL-10 is an immunoregulatory cytokine. Its main function is the limitation and termination of inflammatory responses and the regulation of differentiation and proliferation of several immune cells such as T cells, B cells, natural killer cells, antigen-presenting cells, mast cells, and granulocytes.
Interaction
IL-10R is expressed in monocytes, NK, B and T cells. In addition, Langerhans cells, dermal dendritic cells, eosinophils, mast cells, and endothelial cells can respond to IL-10.
Ligand/Receptor
IL-10R1 and IL-10R2
Biology Area
Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Fiorentino DF, et al. 1989. J Exp Med. 170:2081-2095.
  2. Ho AS, et al. 1993. P. Natl. Acad. Sci. USA. 90:11267-11271.
  3. Hart PH, et al. 1996. J Immunol. 157:3672-3680.
  4. Asadullah K, et al. 2003. Pharmacol Rev. 55:241-269.
  5. Mosser DM and Zhang X. 2008. Immunol Rev. 226:205-218.
  6. Maynard CL and Weaver CT. 2008. Immunol Rev. 226:219-233.
Gene ID
3586 View all products for this Gene ID
UniProt
View information about IL-10 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 06/11/2024

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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