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- Regulatory Status
- RUO
- Other Names
- A Proliferation Inducing Ligand (APRIL), TALL-2, APOL-related leukocyte expressed ligand 2, CD256, member 13, TNF-related death ligand 1, TRDL-1, tumor necrosis factor (ligand) superfamily, ZTNF2
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 439304 | 5 plates | 304€ | ||||
APRIL, or TNFSF13, stands for “A Proliferation-Inducing Ligand.” It is a member of the TNF superfamily of ligands, closely related to BAFF, with which it shares 30% amino acid sequence identity. APRIL is cleaved intracellularly by furin, and is believed to exist mainly as a secreted, soluble form. APRIL exists as a functional homotrimer (total MW = 90 kD). It can bind to two cell-surface receptors: BCMA and TACI, which it shares with BAFF, to exert downstream T- and B-cell regulatory effects. It also possesses a heparin binding domain and has been demonstrated to bind to proteoglycans on the cell surface. APRIL is known for its tumor proliferation effects. It is a potential biomarker, with serum levels elevated for certain cancers, and expression levels elevated in cancer tissues. It is also a potential biomarker for certain autoimmune diseases.
Product DetailsKit Contents
- Kit Contents
-
- Human APRIL/TNFSF13 Capture Antibody (200X)
- Human APRIL/TNFSF13 Detection Antibody (200X)
- Human APRIL/TNFSF13 Lyophilized Standard
- Avidin-HRP (1000X)
- Substrate Solution F
- Coating Buffer A (5X)
- Blocking Buffer C (5X)
- Assay Diluent A (5X)
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
- Sensitivity
- 0.06 ng/mL
- Standard Range
- 0.16 – 10 ng/mL
- Materials Not Included
-
- Microwell plates: BioLegend Cat. No. 423501 is recommended
- Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
- Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
- Plate Sealers: BioLegend Cat. No. 423601 is recommended
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
- Deionized (DI) water
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
Antigen Details
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
- Molecular Family
- Cytokines/Chemokines
- Gene ID
- 8741 View all products for this Gene ID
- UniProt
- View information about APRIL on UniProt.org
Related FAQs
- Do the ELISA MAX™ Deluxe Sets come with plates?
-
No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.
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