FITC anti-human CD338 ABCG2 Antibody anti-CD338

Pricing & Availability

Clone: 5D3 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: ABCG2, CDw338, Bcrp1, MXR
Isotype: Mouse IgG2b, κ
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Product Citations: publications

ABCG2 transfected cells stained with 5D3 FITC

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332014

100 tests

184€

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Description

CD338, known as ABCG2 (BCRP1), is a multi-drug resistance (MDR) family protein that is a member of ATP binding cassette (ABC) transporters. It is highly expressed on primitive ‘side population’ (SP) stem cells. This SP phenotype is based on the efflux of fluorescent dyes such as Rhodamine 123 and Hoechst 33342. The SP cells express low or undetectable levels of CD34. In the bone marrow, about 0.05% population of cells displays the low fluorescence and are highly enriched for repopulating cells. The highest levels of BCRP1 mRNA expression have been seen in KDR+ human stem cells. The expression of ABCG2 appears to be highly conserved as it has been identified in various species.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: This clone is listed under the following US Patent Numbers:
6,313,277; 7,541,437; 7,655,755; 7,741,455
RRID: AB_1134191 (BioLegend Cat. No. 332014)

Antigen Details

Distribution: primitive 'sidepopulation' (SP) stem cells, KDR+ human stem cells
Cell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors, Neural Stem Cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Kim M, et al. 2002. Clin. Cancer Res. 8:228.
2. Scharenber CW, et al. 2002. Blood 99:50712.
3.Goodell MA, et al. 1997. Nat. Med. 3:133745.
4. Bunting KD. 2002. Stem Cells. 20:11.
Gene ID: 9429 View all products for this Gene ID
UniProt: View information about CD338 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Stem Cells and Development

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Other Formats

View All CD338 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-human CD338 (ABCG2)	5D3	FC
FITC anti-human CD338 (ABCG2)	5D3	FC
APC anti-human CD338 (ABCG2)	5D3	FC
PerCP/Cyanine5.5 anti-human CD338 (ABCG2)	5D3	FC
PE/Cyanine7 anti-human CD338 (ABCG2)	5D3	FC
TotalSeq™-C0569 anti-human CD338 (ABCG2)	5D3	PG

Certificate of Analysis

Go To Top Version: 2 Revision Date: 12/17/2013

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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