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FITC anti-mouse Ly-51 Antibody

Pricing & Availability
Clone
6C3 (See other available formats)
Regulatory Status
RUO
Other Names
6C3/BP-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
6C3_FITC_1_102510
C57BL/6 mouse bone marrow cells were stained with B200 APC and Ly-51 (clone 6C3) FITC (top) or rat IgG2a FITC isotype control (bottom) (gated on lymphoid cell population)
  • 6C3_FITC_1_102510
    C57BL/6 mouse bone marrow cells were stained with B200 APC and Ly-51 (clone 6C3) FITC (top) or rat IgG2a FITC isotype control (bottom) (gated on lymphoid cell population)
  • 6C3_FITC_2_102510
Compare all formats See FITC spectral data
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108305 50 µg 75€
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Description

Ly-51 is a 140 kD protein also known as 6C3/BP-1. It is a homodimeric cell-surface glycoprotein with aminopeptidase A (APA) activity. Ly-51 is expressed on B cell progenitors, bone marrow stromal cell lines, thymic dendritic cells, and cortical epithelial cells. Ly-51 expression can be upregulated by IL-7 stimulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/6 mouse Pre-B lymphoma cell line (L1-2) plus Abelson murine leukemia virus-specific cytotoxic T-cell clones
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2 and immunohistochemical staining3-5, 7-8 of acetone-fixed frozen sections.

Application References
  1. Pillemer E, et al. 1984. P. Natl. Acad. Sci. USA 81:4434. (IP)
  2. Hardy RR, et al. 1991. J. Exp. Med. 173:1213. (IP)
  3. Adkins B, et al. 1988. Immunogenetics 27:180. (IHC)
  4. Surh CD, et al. 1992. J. Exp. Med. 176:611. (IHC)
  5. Goverman J, et al. 1997. Immunol. Today 18:204. (IHC)
  6. Dumont-Lagace M, 2014. J. Immunol. 192:2219. PubMed
  7. Kvell K, et al. 2010. PLoS One 5:e10701. (FC, IHC)
  8. Griewank K, et al. 2007. Immunity 27:751. (FC, IHC)
Product Citations
  1. Weng L, et al. 2016. Sci Rep. 6:38632. PubMed
  2. Sitte S, et al. 2012. J Immunol. 188:2677. PubMed
  3. Chou W, et al. 2006. Blood. 108:3005. PubMed
  4. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
  5. Xiao G, et al. 2018. Cell. 173:470. PubMed
  6. Miragaia RJ, et al. 2018. Sci Rep. 8:685. PubMed
  7. Kim JM, et al. 2020. Nat Commun. 11:2289. PubMed
  8. Agarwal P, et al. 2021. Cell Reports. 36(2):109386. PubMed
  9. Su M, et al. 2018. Immunol Cell Biol. 9:2448. PubMed
  10. Wang L, et al. 2020. Nat Commun. 0.654166667. PubMed
  11. Han Y, et al. 2019. J Clin Invest. 130:26. PubMed
RRID
AB_313362 (BioLegend Cat. No. 108305)

Antigen Details

Structure
Aminopeptidase A, 140 kD
Distribution

B progenitors, bone marrow stromal lines, thymic dendritic cells, cortical epithelial cells

Function
Aminopeptidase, may function in IL-7-driven proliferation of B cell precursors
Cell Type
B cells, Dendritic cells, Epithelial cells
Biology Area
Immunology
Antigen References

1. Goverman J, et al. 1997. Immunol. Today 18:204.
2. Morse HC, et al. 1987. J. Exp. Med. 165:920.
3. Sherwood P, et al. 1990. Int. Immunol. 2:399.
4. Lin Q, et al. 1998. J. Immunol. 160:4681.

Gene ID
13809 View all products for this Gene ID
UniProt
View information about Ly-51 on UniProt.org
Go To Top Version: 2    Revision Date: 10/10/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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