GMP PE anti-human CD3 Antibody, CD3, SK7

Pricing & Availability

Clone: SK7 (See other available formats)
Other Names: T3, CD3ε
Isotype: Mouse IgG1, κ
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SK7_GMP_PE_CD3_Antibody_051722 — Typical results from human peripheral blood lymphocytes stained either with SK7 PE used at 5 µL/test (filled histogram) or with an isotype control (open histogram).

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260098

100 tests

248€

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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details

Quality Statement This BioLegend’s GMP-RUO reagent is a fluorochrome-labeled monoclonal antibody single reagent for Research Use Only. Suitable for use in laboratory-developed multicolor flow cytometry tests. Single GMP-RUO products can be used in various multicolor flow cytometry combinations. Laboratory need to establish the GMP-RUO performance characteristics in combination with other reagents in normal and pathological samples.

Technical Data Sheet (pdf)

Product Details

Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.2% (w/v) BSA (origin USA) and a stabilizer.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 25 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^4,5,8, immunofluorescent staining⁶, and Western blotting³.

Antigen Details

Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ, (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK T cells, during thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand/Receptor: Peptide antigen bound to MHC
Cell Type: NKT cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, TCRs
Antigen References: Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.

Beverly P, et al. 1981. Eur. J. Immunol. 11:329.

Lanier L, et al. 1986. J. Immunol. 137:2501.
Gene ID: 916 View all products for this Gene ID
UniProt: View information about CD3 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

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Alexa Fluor® 700 anti-human CD3	SK7	FC
Pacific Blue™ anti-human CD3	SK7	FC
Alexa Fluor® 647 anti-human CD3	SK7	FC
Brilliant Violet 510™ anti-human CD3	SK7	FC
Brilliant Violet 421™ anti-human CD3	SK7	FC
Brilliant Violet 605™ anti-human CD3	SK7	FC
Brilliant Violet 711™ anti-human CD3	SK7	FC
FITC anti-human CD3	SK7	FC
PE anti-human CD3	SK7	FC
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PE/Dazzle™ 594 anti-human CD3	SK7	FC
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TotalSeq™-A0049 anti-human CD3	SK7	PG
PerCP/Cyanine5.5 anti-human CD3	SK7	FC
TotalSeq™-C0049 anti-human CD3	SK7	PG
Spark Blue™ 550 anti-human CD3	SK7	FC
PE/Cyanine7 anti-human CD3	SK7	FC
TotalSeq™-B0049 anti-human CD3	SK7	PG
Alexa Fluor® 660 anti-human CD3	SK7	FC
APC/Fire™ 810 anti-human CD3	SK7	FC
Spark NIR™ 685 anti-human CD3	SK7	FC
PE/Fire™ 640 anti-human CD3	SK7	FC
PE/Fire™ 700 anti-human CD3	SK7	FC
APC anti-human CD3	SK7	FC
PerCP anti-human CD3	SK7	FC
APC/Fire™ 750 anti-human CD3	SK7	FC
GMP FITC anti-human CD3	SK7	FC
PE/Cyanine5 anti-human CD3 Antibody	SK7	FC
GMP PE anti-human CD3	SK7	FC
GMP APC anti-human CD3	SK7	FC
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Spark YG™ 593 anti-human CD3	SK7	FC
GMP PerCP anti-human CD3	SK7	FC
Spark Violet™ 500 anti-human CD3	SK7	FC
Brilliant Violet 650™ anti-human CD3	SK7	FC
Spark Violet™ 500 anti-human CD3	SK7	FC
Spark PLUS UV395™ anti-human CD3	SK7	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 06/08/2022

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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