GoInVivo™ Purified anti-human/mouse/rat CD278 (ICOS) anti-CD278

Pricing & Availability

Clone: C398.4A (See other available formats)
Regulatory Status: RUO
Other Names: Inducible COStimulatory molecule, H4
Isotype: Armenian Hamster IgG
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Product Citations: publications

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313541

5 mg

616€

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313542

25 mg

1892€

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313543

50 mg

2860€

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313544

100 mg

4268€

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313545

500 mg

14740€

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313546

1 g

22880€

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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

ICOS, also known as inducible costimulatory molecule and H4, is a 47-57 kD protein. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS is involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7h/B7RP-1 or B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human, Mouse, Rat

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus, Pig

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Mouse T cell clone D10.G4.1

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 1.0 EU/mg of the protein (< 0.1 pg/µg of the protein) as determined by the LAL test.

Preparation

The GoInVivo™ antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment.

Application Notes

The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, in vitro costimulation of T cell activation^1,3,4, and spatial biology (IBEX)^5,6. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 313512).

Additional Product Notes

GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage.

Application References

Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781. (FC IP Costim)
Yagi J, et al. 2003. J. Immunol. 171:783. (FC)
Arimura Y, et al. 2002. Int. Immunol. 14:555. (Costim)
Arimura Y, et al. 2004. J. Biol. Chem. 279:11408. (Costim)
Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

RRID

AB_2728259 (BioLegend Cat. No. 313541)
AB_2728259 (BioLegend Cat. No. 313542)
AB_2728259 (BioLegend Cat. No. 313543)
AB_2728259 (BioLegend Cat. No. 313544)
AB_2728259 (BioLegend Cat. No. 313545)
AB_2728259 (BioLegend Cat. No. 313546)

Antigen Details

Structure: CD28/CTLA-4, 47-57 kD
Distribution: Activated T cells, subset of thymocytes
Function: Costimulates T cell activation, proliferation, humoral immune response
Ligand/Receptor: B7h/B7RP-1/GL-50
Cell Type: T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781.
2. Hutloff A, et al. 1999. Nature 397:263.
3. Buonfiglio D, et al. 2000. Eur. J. Immunol. 30:3463.
4. Coyle AJ, et al. 2000. Immunity 13:95.
Gene ID: 100048841 View all products for this Gene ID 29851 View all products for this Gene ID 64545 View all products for this Gene ID
UniProt: View information about CD278 on UniProt.org

Documentation

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Other Formats

View All CD278 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC,IP,Costim,SB
Biotin anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
FITC anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
PE anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
APC anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Alexa Fluor® 488 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC,SB
Alexa Fluor® 647 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC,IHC-F
PerCP/Cyanine5.5 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
PE/Cyanine7 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Pacific Blue™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Brilliant Violet 421™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Brilliant Violet 510™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
APC/Cyanine7 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
PE/Dazzle™ 594 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Alexa Fluor® 700 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
APC/Fire™ 750 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Brilliant Violet 785™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Brilliant Violet 605™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Ultra-LEAF™ Purified anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC,IP,Costim
GoInVivo™ Purified anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Brilliant Violet 711™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Brilliant Violet 650™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
TotalSeq™-B0171 anti-human/mouse/rat CD278 (ICOS)	C398.4A	PG
TotalSeq™-C0171 anti-human/mouse/rat CD278 (ICOS)	C398.4A	PG
TotalSeq™-A0171 anti-human/mouse/rat CD278 (ICOS)	C398.4A	PG
Brilliant Violet 750™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
TotalSeq™-D0171 anti-human/mouse/rat CD278 (ICOS) Antibody	C398.4A	PG
PE/Cyanine5 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Spark Red™ 718 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 11/02/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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