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ELISA MAX™ Deluxe Set Human IFN-γ

Pricing & Availability
Regulatory Status
RUO
Other Names
IFN-g ELISA Kit
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Product Citations
publications
human ifn-g_121609
  • human ifn-g_121609
Cat # Size Price Quantity Check Availability Save
430104 5 plates 244€
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430115 10 Plates 469€
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430116 20 Plates 751€
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Description

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!

BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human IFN-γ ELISA MAX™ Capture Antibody (200X)
  • Human IFN-γ ELISA MAX™ Detection Antibody (200X)
  • Human IFN-γ Standard
  • Avidin-HRP (1000X)
  • Substrate Solution D
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Older lots of these products may contain Substrate Solutions A and B instead of Substrate Solution D mentioned on the TDS and the current manual. Please refer to the lot-specific CoA for the list of the reagents included in the lot and follow the ELISA procedure provided there.

Product Details

Verified Reactivity
Human
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application Notes

The assay kits will no longer include ELISA microwell plates. Recommended microwell plates can be purchased separately under Cat. No. 423501. Please contact your BioLegend sales representative for bulk pricing quotations.

Application References
  1. Ye Z, et al. 2008. Infect Immun. 75:2541. PubMed
  2. Zhang Y, et al. 2010. Infect Immun. 78:2329. PubMed
  3. Peng J, et al. 2005. J. Immunother. 28:599. PubMed
  4. Bendersky A, et al. 2012. J. Immunol. 188:4349. PubMed
  5. Boggio E, et al. 2012. Hum Immunol. 73:585. PubMed
  6. Ankrum JA, et al. 2014. Sci Rep. 4:4645. PubMed
  7. Wu Z, et al. 2015. J Virol. 89:2906. PubMed
Product Citations
  1. Cordoba S, et al. 2021. Nat Med. 27:1797. PubMed
  2. Heitzeneder S, et al. 2022. Cancer Cell. 40:53. PubMed
  3. Shemesh A, et al. 2022. J Exp Med. 219: . PubMed
  4. Bigenwald C, et al. 2023. Oncoimmunology. 12:2163785. PubMed
  5. Sheng YR, et al. 2022. Cell Mol Life Sci. 79:173. PubMed
  6. Labanieh L, et al. 2022. Cell. 185:1745. PubMed
  7. Scurr MJ, et al. 2022. Nat Commun. 13:5422. PubMed
  8. Wu Z, et al. 2015. J Virol. 89:2906. PubMed
  9. Klement JD, et al. 2018. J Clin Invest. 128:5549. PubMed
  10. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  11. Leland P, et al. 2023. J Transl Med. 21:367. PubMed
  12. Loh HP, et al. 2023. MAbs. 2231129:15. PubMed
  13. Bröker K, et al. 2019. J Transl Med. 17:6. PubMed
  14. Pires D, et al. 2021. Front Immunol. 12:742822. PubMed
  15. Kilcollins A, et al. 2016. J Immunol. 197: 419 - 428. PubMed
  16. Meryk A et al. 2019. Cell reports. 26(10):2681-2691 . PubMed
  17. Singhal J, et al. 2022. Front Cell Infect Microbiol. 12:878136. PubMed
  18. Wang H, et al. 2021. J Immunother Cancer. 9:. PubMed
  19. Singh N, et al. 2018. PLoS One. 13:e0199817. PubMed
  20. Boggio E, et al. 2012. Hum Immunol. 73:585. PubMed
  21. Booty MG, et al. 2022. J Immunol. 208:929. PubMed
  22. Bendersky A, et al. 2012. J Immunol. 188:4349. PubMed
  23. Ye Z, et al. 2008. Infect Immun. 76:2541. PubMed
  24. Ollé Hurtado M, et al. 2019. PLoS One. 14:e0216373. PubMed
  25. You Y, et al. 2021. Mol Med Rep. 24:. PubMed
  26. Ankrum J, et al. 2014. Sci Rep. 4:4645. PubMed
  27. Karampetsou M, et al. 2016. J Immunol. 196: 4915 - 4924. PubMed
  28. Aldeghaither DS, et al. 2019. Cancer Immunol Res. 7:230. PubMed
  29. Kofla G, et al. 2022. Oncoimmunology. 11:2068109. PubMed
  30. Godbersen C, et al. 2017. Mol Cancer Ther. 16:1335. PubMed
  31. Sp N, et al. 2021. Int J Mol Sci. 22:. PubMed
  32. Hirschberger S, et al. 2022. Front Med (Lausanne). 9:923502. PubMed
  33. Liu CA, et al. 2022. Cancers (Basel). 14:. PubMed
  34. Madison BB, et al. 2022. Mol Ther Nucleic Acids. 29:979. PubMed
  35. Feng Y, et al. 2022. Life (Basel). 12:. PubMed
  36. Fernandes RA, et al. 2020. Nature. 779:586. PubMed
  37. Bliska Y 2010. Infect Immun. 78:2359. PubMed
  38. Leung EYL, et al. 2020. Mol Ther Oncolytics. 16:289. PubMed
  39. Wu Z, et al. 2019. Microorganisms. 0.670833333. PubMed
  40. Trinklein ND, et al. 2019. MAbs. 11:639. PubMed
  41. Xie X, et al. 2021. Front Immunol. 12:625808. PubMed
  42. Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
  43. Pires D, et al. 2021. Front Immunol. 12:647728. PubMed
  44. Subramanian N, et al. 2017. J Gen Virol. 98(12). PubMed
  45. Iraqi M, et al. 2022. Int J Mol Sci. 23:. PubMed
  46. Kang CH, et al. 2020. Int J Mol Sci. 21:00. PubMed
  47. Zhang X, et al. 2019. Hematol Oncol. 37(3):270. PubMed
  48. Singh SS, et al. 2022. Clin Transl Immunology. 11:e1396. PubMed
  49. Pierini S, et al. 2020. JCI Insight. 5:00. PubMed
  50. Dammermann W, et al. 2017. J Virol Methods. 10.1016/j.jviromet.2017.07.011. PubMed
  51. Lei W, et al. 2022. Blood Cancer J. 12:35. PubMed
  52. Wang E, et al. 2015. Cancer Immunol Res. 3: 815 - 826. PubMed
  53. Kunyanee C, et al. 2016. PLoS One. 11: 0160741. PubMed
  54. Lucchesi D, et al. 2020. Arthritis Rheumatol. 1559:72. PubMed
  55. Singh N, et al. 2017. Mol Immunol. 85:111. PubMed
Sensitivity
4 pg/mL
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Antigen Details

Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Type
Tregs
Biology Area
Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 6    Revision Date: 07/15/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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