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- Regulatory Status
- RUO
- Other Names
- CCL1 (I-309), CCL13 (MCP-4), CCL22 (MDC), CCL7 (MCP-3), CCL24 (Eotaxin-2), CCL19 (ELC), CXCL13 (BLC), CCL8 (MCP-2), CX3CL1 (Fractalkine) CXCL12 (SDF-1), CXCL2 (GRO-β)
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Chemotactic cytokines or chemokines play pivotal roles in various processes such as immune surveillance, organ development, angiogenesis, and immune responses. Expression profiling of chemokines, especially those involved in inflammation and immune disorders, is important in achieving a deeper understanding of disease states.
The Human Proinflammatory Chemokine Panel 2 (11-plex) is a multiplex bead-based assay panel, using fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 12 human chemokines, including CCL1 (I-309), CCL13 (MCP-4), CCL22 (MDC), CCL7 (MCP-3), CCL24 (Eotaxin-2), CCL19 (ELC), CXCL13 (BLC), CCL8 (MCP-2), CX3CL1 (Fractalkine), CXCL12 (SDF-1), and CXCL2(GRO-β). This assay panel provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. The panel has been validated by detecting expected changes in biological samples.
The panel has been validated for use on cell culture supernatant and serum samples. Use with caution for plasma samples.
|
Cat # |
Plex Size |
Targets |
Sample Type |
Dilution Factor |
|
741157, 741158 |
12-plex |
CCL1, CCL13, CCL22, CCL7, CCL24, CCL19, CX CL13, CCL8, CX3CL1, CXCL12, CXCL2, CCL18 |
Tissue Culture |
Varies |
|
741182, 741183 |
11-plex |
CCL1, CCL13, CCL22, CCL7, CCL24, CCL19, CX CL13, CCL8, CX3CL1, CXCL12, CXCL2 |
Tissue Culture |
Varies |
|
Serum/Plasma |
2X |
|||
|
741184, 741185 |
1-plex |
CCL18 |
Tissue Culture |
Varies |
|
Serum/Plasma |
50X |
Please visit www.biolegend.com/legendplex for more information on how to mix and match within the panel.
Product Details
Kit Contents
- Kit Contents
-
- Setup Beads: PE Beads
- Setup Beads: Raw Beads
- Set of capture beads, 13X
- HU Proinflam. Chemokine Panel 2 Detection Antibodies
- HU Proinflam. Chemokine Panel 2 Standard Cocktail, Lyophilized
- LEGENDplex™ SA-PE
- LEGENDplex™ Matrix A, Lyophilized
- LEGENDplex™ Lyophilized Standard Reconstitution Buffer
- LEGENDplex™ Assay Buffer
- LEGENDplex™ Wash Buffer, 20X
- Plate Sealers
- V-bottom plate
Product Details
- Verified Reactivity
- Human
- Application
-
Multiplex
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here. - Materials Not Included
-
- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes
- Vortex Mixer
- Sonicator
- Aluminum Foil
- Absorbent Pads or Paper Towels
- Plate Shaker
- Tabletop Centrifuges
- A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
- Centrifuge with a swinging bucket adaptor for microtiter plates (if using V-bottom plates)
- 1.1 mL polypropylene micro FACS tubes, in 96-tube rack
Antigen Details
- Gene ID
- 6346 View all products for this Gene ID 6357 View all products for this Gene ID 6367 View all products for this Gene ID 6354 View all products for this Gene ID 6369 View all products for this Gene ID 6363 View all products for this Gene ID 10563 View all products for this Gene ID 6355 View all products for this Gene ID 6376 View all products for this Gene ID 6387 View all products for this Gene ID 2920 View all products for this Gene ID 6362 View all products for this Gene ID
Related FAQs
- If I don't have a vacuum, how do I remove the liquid from my plate?
-
If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
-
Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
-
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
-
LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
-
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.
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