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- Regulatory Status
- RUO
- Other Names
- OPG, OPN, PDGF-BB, ALPL, ACP5, Leptin, RANKL (TRANCE), TNF-α, IL-6, PTH, IL-1β, BMP-2, DKK-1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Bone metabolism is a complex process of bone formation and resorption regulated by numerous hormones, steroids, growth factors, and cytokines. Bone formation is mediated by specialized cells called osteoblasts, while resorption is carried out via osteoclasts. Homeostatic imbalance may lead to various diseases such as: osteoporosis, osteopetrosis, Paget’s disease, rickets, and renal osteodystrophy.
The LEGENDplex™ Human Bone Metabolism Panel V02 is a multiplex bead-based assay suitable for use on various flow cytometers. This panel allows simultaneous quantification of 13 human proteins, including Osteoprotegerin (OPG), Osteopontin (OPN), PDGF-BB, Alkaline Phosphatase Liver/Bone/Kidney (ALPL), Acid Phosphatase 5 Tartrate Resistant (ACP5), Leptin, RANKL (TRANCE), Tumor Necrosis Factor Alpha (TNF-α), Interleukin 6 (IL-6), Parathyroid Hormone (PTH), IL-1β, Bone Morphogenetic Protein 2 (BMP-2), and Dickkopf WNT Signaling Pathway Inhibitor 1 (DKK-1). This assay provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. The panel has been validated for use with serum, plasma, and cell culture supernatants.
Kit Contents
- Kit Contents
-
- Setup Beads: PE Beads
- Setup Beads: Raw Beads
- LEGENDplex™ Human Bone Metabolism Panel V02 Premixed Beads
- LEGENDplex™ Human Bone Metabolism Panel V02 Det. Ab
- LEGENDplex™ Human Bone Metabolism Panel V02 Standard
- LEGENDplex™ Matrix A, Lyophilized
- LEGENDplex™ SA-PE
- LEGENDplex™ Assay Buffer
- LEGENDplex™ Wash Buffer, 20X
- Plate Sealers
- Filter Plate
Product Details
- Verified Reactivity
- Human
- Application
-
Multiplex - Panel
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here. - Materials Not Included
-
- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes
- Vortex Mixer
- Sonicator
- Aluminum Foil
- Absorbent Pads or Paper Towels
- Plate Shaker
- Tabletop Centrifuges
- A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
- 1.1 mL polypropylene micro FACS tubes, in 96-tube rack
- Manual
Antigen Details
- Gene ID
- 5155 View all products for this Gene ID 22943 View all products for this Gene ID 7124 View all products for this Gene ID 3553 View all products for this Gene ID 4982 View all products for this Gene ID 6696 View all products for this Gene ID 249 View all products for this Gene ID 54 View all products for this Gene ID 3952 View all products for this Gene ID 8600 View all products for this Gene ID 3569 View all products for this Gene ID 5741 View all products for this Gene ID 650 View all products for this Gene ID
Related FAQs
- If I don't have a vacuum, how do I remove the liquid from my plate?
-
If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
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Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
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The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
-
LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
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Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.
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