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MojoSort on Columns™ - Human NK Cell Isolation Kit

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Regulatory Status
RUO
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A. Human-NK-Cell-Isolation-Kit_042825
Untouched NK cells were isolated from fresh human whole blood PBMCs using the MojoSort on Columns™ - Human NK Cell Isolation Kit with BioLegend’s Large Columns (Cat. No. 480178). The negatively selected cells were then stained with APC anti-human CD3 (clone UCHT1) (Cat. No. 300411) and PE anti-human CD56 (clone 5.1H11) (Cat. No. 362507). The cells were then analyzed by flow cytometry gated on live CD45+ cells.
  • A. Human-NK-Cell-Isolation-Kit_042825
    Untouched NK cells were isolated from fresh human whole blood PBMCs using the MojoSort on Columns™ - Human NK Cell Isolation Kit with BioLegend’s Large Columns (Cat. No. 480178). The negatively selected cells were then stained with APC anti-human CD3 (clone UCHT1) (Cat. No. 300411) and PE anti-human CD56 (clone 5.1H11) (Cat. No. 362507). The cells were then analyzed by flow cytometry gated on live CD45+ cells.
  • B. Human-NK-Cell-Isolation-Kit_2_042825
    Untouched NK cells were isolated from frozen human leukopak PBMCs using the MojoSort on Columns™ - Human NK Cell Isolation Kit with a BioLegend’s Large Columns (Cat. No. 480178). The negatively selected cells were then stained with PE anti-human CD56 (clone 5.1H11) (Cat. No. 362507) and APC anti-human CD3 (clone UCHT1) (Cat. No. 300411). The cells were then analyzed by flow cytometry gated on live CD45+ cells.
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480186 100 tests 404€
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Description

MojoSort on Columns™ is BioLegend’s product line for column-based cell separation. This portfolio offers total solutions for column system setup as well as column-optimized formulations for reagents and kits.

This kit is designed for negative isolation of human natural killer cells (NK cells) from PBMCs harvested from whole blood and leukopaks. Non-NK cells are magnetically labeled by incubating your sample with the biotin antibody cocktail followed by magnetic Streptavidin Nanobeads. Once applied to our MojoSort on Columns™ System, the magnetically labeled cells are retained in the column matrix, while the untouched NK cells flow through the column and are collected into a clean tube. The depleted/labeled non-NK cells can be eluted from the column matrix for use as a control sample. 

Product Details
Technical Data Sheet (pdf)

Kit Details

Kit Contents

For Cat# 480186:

  • 1 vial containing 1 mL of Biotin Antibody Cocktail
  • 1 vial containing 1 mL of Streptavidin Nanobeads 

Product Details

Verified Reactivity
Human
Formulation
Cocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.

Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
The antibodies were purified by affinity chromatography and conjugated with biotin under optimal conditions.

Streptavidin Nanobeads: Streptavidin-coated magnetic beads
Storage & Handling
Antibody cocktail and Streptavidin Nanobeads should be stored upright and undiluted between 2°C and 8°C. For lot-specific expiration dates, please refer to the Certificate of Analysis which can be found here.
Application

Cell Separation – Neg (Quality tested)
Recommended Usage

10 µL of antibody cocktail for 1 x 107 cells in 100 µL of buffer.
10 µL of Streptavidin Nanobeads for 1 x 107 cells in 100 µL of buffer.

Application Notes

This kit is designed for negative isolation of natural killer cells (NK cells) from human PBMCs derived from whole blood or leukopaks. This kit is optimized for use with a column-based separation system such as MojoSort on Columns™ - Large Columns (Cat. No. 480178), Multistand (Cat. No. 480177) and Column Separator (Cat. No. 480179). This kit has also been tested for compatibility with columns from other suppliers. Please contact BioLegend Technical Service (tech-biolegend@revvity.com) for more information and guidance on compatibility with 3rd party columns and column systems. This product is designed research use only (RUO).

Important Note: This kit is not suitable for use with our handheld magnets. If you're using handheld magnets, we recommend the MojoSort™ Human NK Cell Isolation Kit (Cat. No. 480053).

Test sizes/starting cell capacity:

  • One test is defined as cell separation from 1x107 human whole blood PBMCs or leukopak-derived PBMCs.
  • The 100 tests size provides enough reagents for approximately 1x109 cells.

Each lot has been individually optimized. Do not mix and match components from different lots of different kits

Antigen Details

Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/08/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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