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PE anti-human CD275 (B7-H2, ICOSL) Antibody

Pricing & Availability
Clone
W21036I (See other available formats)
Regulatory Status
RUO
Other Names
CD275, ICOSL, B7H2, ICOS-L, B7RP-1, B7-RP1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W21036I_PE_CD275_Antibody_052623
Human peripheral blood lymphocytes were surface stained with anti-human CD19 APC and anti-human CD275 (B7-H2, ICOSL) (clone W21036I) PE (left) or rat IgG2a, κ PE isotype control (right). Data shown were gated on lymphocyte population.
  • W21036I_PE_CD275_Antibody_052623
    Human peripheral blood lymphocytes were surface stained with anti-human CD19 APC and anti-human CD275 (B7-H2, ICOSL) (clone W21036I) PE (left) or rat IgG2a, κ PE isotype control (right). Data shown were gated on lymphocyte population.
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387503 25 tests 88€
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387504 100 tests 200€
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Description

B7-H2, a member of the B7 family and the immunoglobulin superfamily, is a 40 kD protein also known as B7RP-1, B7h, B7-H2, GL50 and ICOS Ligand. Human B7-H2 is expressed by B lymphocytes, activated monocytes/macrophages, and dendritic cells. B7-H2 binds to a CD28-like receptor, inducible costimulator molecule (ICOS, AILIM, CRP-1), which is expressed by activated T cells. The interaction of ICOS with B7-H2 plays an important role in the T cell costimulation pathway.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human B7-H2 recombinant protein (19-258 aa)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone W21036I completely blocks clone 9F.8A4 but does not block clone 2D3.

RRID
AB_2941575 (BioLegend Cat. No. 387503)
AB_2941575 (BioLegend Cat. No. 387504)

Antigen Details

Structure
Ig superfamily, B7 family, 40 kD
Distribution

B cells and dendritic cells

Function
Binds ICOS on antigen-activated T cells and mediates proliferation and cytokine production
Interaction
B cells, Dendritic cells
Ligand/Receptor
ICOS
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Wang S, et al. 2002. J Biol Chem. 96:2808.
  2. Wong SC, et al. 2003. Blood. 102:1831.
Gene ID
23308 View all products for this Gene ID
UniProt
View information about CD275 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 05/26/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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