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PE anti-human SUSD2 Antibody

Pricing & Availability
Clone
W5C5 (See other available formats)
Regulatory Status
RUO
Other Names
Mesenchymal Stem Cells (MSC), Sushi domain containing 2 (SUSD2)
Isotype
Mouse IgG1, κ
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Product Citations
publications
W5C5_PE_A_020808
Human cervical cancer cell line, HeLa, stained with W5C5 PE
  • W5C5_PE_A_020808
    Human cervical cancer cell line, HeLa, stained with W5C5 PE
  • W5C5_PE_B_020808
    Human cervical cancer cell line, HeLa, stained with W5C5 PE
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327406 100 tests 254€
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Description

The W5C5 antibody reacts with SUSD2 (sushi domain containing 2) which is expressed by bone marrow mesenchymal stem cells, vessel-surrounding smooth muscle cells, and a few neoplastic cell lines. SUSD2 is a recently identified type I transmembrane protein of 820 amino acids consisting of a large extracellular region containing a Somatomedin B (SMB), an Adhesion-associated domain in MUC4 and Other Proteins (AMOP), a von Willebrand factor (vWF), and a F1c Sushi domain. Mesenchymal stem cells (MSCs) were originally named bone marrow stromal cells. They are a population of adult stem cells with a large capacity for self-renewal and multipotency for differentiation into a variety of cell types including osteoblasts, chondrocytes, myocytes, adipocytes, β-pancreatic islets cells, and certain neuronal cells. MSCs reside in many cells and tissues, such as bone marrow, placenta, adipose tissue, adult peripheral blood, fetal blood, skin, liver, and lung. Besides their plasticity for tissue repair, MSCs also exhibit powerful immunosuppressive activity and play an important role in supporting hematopoiesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Retinoblastoma cell line (Weri-RB-1 cells).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence2.

Application References
  1. Masuda H, et al. 2012. Cell Transplant. 21:2201.
  2. Sivasubramaniyan K, et al. 2013. Stem Cells Dev. 22:1944. PubMed (IF)
  3. Su K, et al. 2014. Acta Biomater. 10:5012. PubMed
Product Citations
  1. Yu L, et al. 2020. Cell Stem Cell. 28(3):550-567.e12. PubMed
  2. Patani H, et al. 2020. Nat Commun. 3.007638889. PubMed
  3. Rohani L, et al. 2020. Commun Biol. 0.466666667. PubMed
  4. Ulrich D, et al. 2014. Hum Reprod. 29:1895. PubMed
  5. Bredenkamp N, et al. 2019. Stem Cell Reports. 1.29375. PubMed
  6. Aints A, et al. 2019. Sci Rep. 9:12562. PubMed
  7. Dong C, et al. 2022. Nat Commun. 13:2548. PubMed
  8. Miller SA, et al. 2021. Cancer Res. 81:3791. PubMed
  9. Huang X, et al. 2021. Nat Commun. 12:5123. PubMed
  10. Sivasubramaniyan K, et al. 2013. Stem Cells Dev. 22:1944. PubMed
  11. Khan SA, et al. 2021. Cell Reports. 35(11):109233. PubMed
  12. Chen C, et al. 2022. iScience. 25:103645. PubMed
RRID
AB_940654 (BioLegend Cat. No. 327406)

Antigen Details

Distribution

Bone marrow mesenchymal stem cells

Cell Type
Mesenchymal cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Buhring HJ, et al. 2007. Ann. N. Y. Acad. Sci. 1106:262.
2. Jackson L, et al. 2007. J. Postgrad. Med. 53:121.
3. Krampera M, et al. 2003. Blood 101:3722.
4. Ball LM, et al. 2007. Blood 110:2764.

Gene ID
56241 View all products for this Gene ID
UniProt
View information about SUSD2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 04/22/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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