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PE anti-marmoset CD8 Antibody

Pricing & Availability
Clone
6F10 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Leu2
Isotype
Mouse IgG1
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Product Citations
publications
6F10_PE_MarmosetCD8_Antibody_FCC052413
Marmoset peripheral blood lymphocytes were stained with CD8 (clone 6F10) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • 6F10_PE_MarmosetCD8_Antibody_FCC052413
    Marmoset peripheral blood lymphocytes were stained with CD8 (clone 6F10) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
See PE spectral data
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250304 100 µg 210€
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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Common Marmoset
Reported Reactivity
Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Marmoset mononuclear cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 6F10 antibody has been reported to cross-react with lymphocytes from squirrel monkeys but not from cynomolgus monkeys or humans.

Additional reported applications for the relevant formats include: iimmunohistochemical staining of frozen spleen sections1, Western blotting1, immunoprecipitation1.

Application References
  1. Ito R, et al. 2008. Immunol. Lett. 121:116. (IHC, IP, WB)
Product Citations
  1. Gordeychuk IV, et al. 2019. Acta Naturae. 10:63. PubMed
  2. Wang Y, et al. 2021. Front Microbiol. 12:701566. PubMed
RRID
AB_2562122 (BioLegend Cat. No. 250304)

Antigen Details

Structure
Ig superfamily, homodimer or heterodimer with CD8β
Distribution

Majority of thymocytes, T cell subset, NK cells

Function
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor
MHC Class I molecules
Cell Type
NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
100387674 View all products for this Gene ID
UniProt
View information about CD8 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 06/04/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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