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PE anti-mouse CD226 (DNAM-1) Antibody

Pricing & Availability
Clone
10E5 (See other available formats)
Regulatory Status
RUO
Other Names
DNAM-1, PTA1 (platelet and T cell activation antigen 1), TLISA1, LFA-1 associated Molecule PTA-1
Isotype
Rat IgG2b, κ
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Product Citations
publications
10E5_PE_090808.jpg
C57BL/6 splenocytes stained with CD8 (53-6.7) FITC and 10E5 PE
  • 10E5_PE_090808.jpg
    C57BL/6 splenocytes stained with CD8 (53-6.7) FITC and 10E5 PE
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128805 25 µg 98€
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128806 100 µg 259€
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Description

CD226 (DNAM-1) is constitutively expressed on native CD8+ cells and on CD4+ T cells, macrophages and NK cells.This antibody (10E5) was reported to bind about 40% of inactivated CD4+ cells and binds only to differentiated Th1 cells, but not to Th2 or Th0 cells.It is also reported to suppress antigen-specific T cell expansion and EAE (experimental allergic encephalitis) mediated by Th1 cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Th1 polarized T cell clones
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References
  1. Dardalhon V, et al. 2005. J. Immunol. 175:1558
  2. Huang Z, et al. 2011. J Immunol. 186:3421. PubMed
Product Citations
  1. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  2. Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed
  3. Wang YT, et al. 2020. Nat Microbiol. 0.397222222. PubMed
  4. Yasuma K, et al. 2016. PLoS Pathog. 12: 1005372. PubMed
  5. Lu YJ, et al. 2021. Cell Rep. 36:109696. PubMed
  6. Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed
  7. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  8. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  9. Huang X, et al. 2021. Immunity. . PubMed
RRID
AB_1186119 (BioLegend Cat. No. 128805)
AB_1186119 (BioLegend Cat. No. 128806)

Antigen Details

Structure
65 kd glycoprotein of a member of Ig-superfamily containing 2 immunologlobulin-like domains.
Distribution

Expressed on majority of CD4+ and CD8+ T cells, NK cells, monocytes/macrophages and a subset of B cells and thymocytes. Reported to be specifically expressed on the surface of Th1 cells, but downregulated in Th2 cells.

Function
Mediates cellular adhesion, involved in LFA-1 costimulatory signal for T cell differentiation and proliferation
Ligand/Receptor
CD112 and CD155
Cell Type
B cells, Macrophages, Monocytes, NK cells, T cells, Th1
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1.Tahara Hanaoka S, et al. 2006. Blood 107:1491
2. Shibuya K, et al. 2003. J. Exp. Med. 198:1829

Gene ID
225825 View all products for this Gene ID
UniProt
View information about CD226 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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