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PE anti-mouse CD4 Antibody

Pricing & Availability
Clone
RM4-5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1_RM4-5_PE_030206
C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • 1_RM4-5_PE_030206
    C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • 2_RM4-5_PE_CD4_Antibody_2_040122
    C57BL/6 mouse splenocytes were stained with CD4 (clone RM4-5) PE (solid line) or rat IgG2b, κ PE isotype control (dashed line).
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100511 50 µg 20€
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100512 200 µg 72€
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4+ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4+ cells1, and immunohistochemistry of acetone-fixed frozen tissue sections2,3,11 and paraffin-embedded sections11. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100575 and 100576).

Application References
  1. Kruisbeek AM. 1991. In Curr. Protocols Immunol. pp. 4.1.1-4.1.5. (Block, Deplete)
  2. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  3. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045.
  4. Muraille E, et al. 2003. Infect. Immun. 71:2704. (IHC)
  5. León-Ponte M, et al. 2007. Blood 109:3139. (FC)
  6. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. (FC)
  7. Matsumoto M, et al. 2007.J. Immunol.178:2499. PubMed
  8. Shigeta A, et al. 2008. Blood 112:4915. PubMed
  9. Zaborsky N, et al. 2010. J. Immunol. 184:725. PubMed
  10. Rodrigues-Manzanet R, et al. 2010. P. Natl Acad Sci USA 107:8706. PubMed
  11. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
Product Citations
  1. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
  2. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
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  4. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
  5. Sugiura A, et al. 2022. Immunity. 55:65. PubMed
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  11. Seclì L, et al. 2023. J Immunother Cancer. 11:. PubMed
  12. Karagiannis F, et al. 2020. Immunity. 52(4):620-634. PubMed
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  19. Galeano Niño JL, et al. 2020. J Cell Sci. . PubMed
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  21. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  22. Ma C, et al. 2018. Science. 360:eaan5931. PubMed
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  24. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
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  26. Mohan J, et al. 2013. J Exp Med. 210:2403. PubMed
  27. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  28. Jeong SH, et al. 2021. Nat Commun. 12:4405. PubMed
  29. Alshetaiwi H, et al. 2020. Sci Immunol. 5:00. PubMed
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  36. Cook KD, et al. 2015. Immunity. 43:703-714. PubMed
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  59. Tang S, et al. 2022. Virol J. 19:32. PubMed
  60. Liang K, et al. 2021. Clin Transl Immunology. 10:e1293. PubMed
  61. Li J, et al. 2021. Cell Rep. 37:110124. PubMed
  62. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  63. Chen LH, et al. 2021. Sci Rep. 11:19478. PubMed
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RRID
AB_312714 (BioLegend Cat. No. 100511)
AB_312714 (BioLegend Cat. No. 100512)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 01/29/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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