- Clone
- QA20A64 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Killer cell lectin-like receptor G1 (KLRG1), mast cell function-associated antigen (MAFA or 2F1-Ag)
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
164903 | 25 µg | 86€ | ||||
164904 | 100 µg | 240€ |
Killer cell lectin-like receptor G1 (KLRG1) is the mouse homolog of the rat mast cell function-associated antigen (MAFA or 2F1-Ag). KLRG1 is a type II membrane glycoprotein that was first identified on the surface of rat mast cell line RBL-2H3. It is composed of a homodimer of glycosylated 30-38 kD subunits. Mouse and human homologs of KLRG1 are expressed by subsets of NK cells and lymphokine-activated killer (LAK) cells but not mast cells. KLRG1 is also expressed on subsets of CD8+ and CD4+ cells, including CD4+ and CD8+ effector/memory cells, potent regulatory CD4+ T cells. KLRG1 may be involved in regulating NK cell homeostasis. KLRG1 was found to recognize cadherins and thus inhibit immune responses by regulating the effector function and the developmental processes of NK and T cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Clone QA20A64 completely block the binding of reported clone 2F1/KLRG1 on target cells.
- RRID
-
AB_2936557 (BioLegend Cat. No. 164903)
AB_2936557 (BioLegend Cat. No. 164904)
Antigen Details
- Structure
- KLRG1 is an inhibitory lectin-like type II transmembrane receptor containing a cytoplasmic motif similar to ITIM, expressed mainly as a homodimeric molecule consisting of two N-glycosylated subunits of approximately 30-38 kD.
- Distribution
-
Subsets of NK cells, lymphokine-activated killer (LAK) cells and subsets of CD8+ and CD4+ cells.
- Function
- Regulates NK cell proliferation, maturation, and homeostasis. Inhibits immune responses by regulating the effector function and the developmental processes of NK and T cells.
- Interaction
- NK cells, T cells, Tregs
- Ligand/Receptor
- E-cadherin
- Cell Type
- NK cells, T cells
- Biology Area
- Immunology, Innate Immunity
- Antigen References
-
- Grundemann C, et al. 2006. J. Immunol. 176:1311-5.
- Blaser C, et al. 1998. J. Immunol. 161:6451-4.
- Huntington ND, et al. 2007. J. Immunol. 178:4764-70.
- Voehringer D, et al. 2001. J. Immunol. 167:4838-43.
- Gene ID
- 10219 View all products for this Gene ID
- UniProt
- View information about KLRG1 on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All KLRG1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse KLRG1 (MAFA) Recombinant Antibody | QA20A64 | FC |
PE anti-mouse KLRG1 (MAFA) Recombinant Antibody | QA20A64 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-mouse KLRG1 (MAFA) Recombinant Antibody
C57BL/6 mouse splenocytes stained with anti-mouse NK-1.1 APC... -
PE anti-mouse KLRG1 (MAFA) Recombinant Antibody
C57BL/6 mouse splenocytes stained with anti-mouse NK-1.1 APC...
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