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PE anti-SAMHD1 Antibody

Pricing & Availability
Clone
W19081C (See other available formats)
Regulatory Status
RUO
Other Names
SAM and HD Domain Containing Deoxynucleoside Triphosphate, Triphosphohydrolase 1, Monocyte Protein 5, Aicardi-Goutieres Syndrome 5, Sterile Alpha Motif and Histidine Aspartate Domain-Containing Protein 1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A_W19081C_PE_SAMHD1_Antibody_081624
Human peripheral blood mononuclear cells were surface stained with anti-human CD3 (clone UCHT1) Pacific Blue™. Cells were then fixed and permeabilized with True-Phos™ Perm Buffer Set (Cat. No. 425401), then intracellularly stained with anti-SAMHD1 (clone W19081C) PE (left) or rat IgG2a, κ PE isotype control (right).
  • A_W19081C_PE_SAMHD1_Antibody_081624
    Human peripheral blood mononuclear cells were surface stained with anti-human CD3 (clone UCHT1) Pacific Blue™. Cells were then fixed and permeabilized with True-Phos™ Perm Buffer Set (Cat. No. 425401), then intracellularly stained with anti-SAMHD1 (clone W19081C) PE (left) or rat IgG2a, κ PE isotype control (right).
  • B_W19081C_PE_SAMHD1_Antibody_2_070224
    THP1 cells were fixed and permeabilized using True-Phos™ Perm Buffer Set (Cat. No. 425401), then intracellularly stained with anti-SAMHD1 (clone W19081C) PE (closed histogram) or rat IgG2a, κ PE isotype control (open histogram).
See PE spectral data
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941003 25 tests 144€
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941004 100 tests 360€
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Description

SAMHD1 is a host defense factor involved in the innate immune response to viral infections, including HIV-1. Through its dNTPase activity, SAMHD1 disrupts viral genome replication by depleting available pools of deoxynucleotide triphosphates (dNTPs) for viral reverse transcription in dendritic and myeloid cells. In addition to its role in innate immune defense, SAMHD1 also plays an essential role in preserving genome stability through maintaining balanced dNTP pools. Independently of its dNTPase activity, SAMHD1 is required for resection of stalled, gapped, or reversed DNA replication forks by stimulating the exonuclease activity of MRE11. SAMHD1-deficient cells show increased dNTPs and genome instability, and an increased accumulation of G1-positive cells. These cells also accumulate single-strand DNA fragments in the cytosol, leading to the induction of pro-inflammatory type 1 interferons through the cGAS-STING pathway. Mutations in the SAMHD1 are found in many cancers, as well as the severe congenital inflammatory disease Aicardi-Goutieres syndrome.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human SAMHD1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone W19081C is not recommended for immunocytochemistry applications due to non-specific staining.

Additional Product Notes

Clone W19081C can be used with Cyto-Fast™ Fix/Perm Buffer or True-Nuclear™ Transcription Factor Buffer. However, True-Phos™ Perm Buffer is recommended for optimal staining results.

Antigen Details

Structure
SAMHD1 is a 626 amino acid protein with a predicted molecular weight of 72 kD.
Distribution

Ubiquitously expressed/Nucleus

Function
Innate immune response, DNA replication and repair
Cell Type
Dendritic cells, Monocytes
Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Innate Immunity
Molecular Family
Enzymes and Regulators
Antigen References
  1. Laguette N, et al. 2011. Nature. 474:654.
Gene ID
25939 View all products for this Gene ID
UniProt
View information about SAMHD1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 07/02/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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