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- Clone
- W19081C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SAM and HD Domain Containing Deoxynucleoside Triphosphate, Triphosphohydrolase 1, Monocyte Protein 5, Aicardi-Goutieres Syndrome 5, Sterile Alpha Motif and Histidine Aspartate Domain-Containing Protein 1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood mononuclear cells were surface stained with anti-human CD3 (clone UCHT1) Pacific Blue™. Cells were then fixed and permeabilized with True-Phos™ Perm Buffer Set (Cat. No. 425401), then intracellularly stained with anti-SAMHD1 (clone W19081C) PE (left) or rat IgG2a, κ PE isotype control (right). -
THP1 cells were fixed and permeabilized using True-Phos™ Perm Buffer Set (Cat. No. 425401), then intracellularly stained with anti-SAMHD1 (clone W19081C) PE (closed histogram) or rat IgG2a, κ PE isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 941003 | 25 tests | 144€ | ||||
| 941004 | 100 tests | 360€ | ||||
SAMHD1 is a host defense factor involved in the innate immune response to viral infections, including HIV-1. Through its dNTPase activity, SAMHD1 disrupts viral genome replication by depleting available pools of deoxynucleotide triphosphates (dNTPs) for viral reverse transcription in dendritic and myeloid cells. In addition to its role in innate immune defense, SAMHD1 also plays an essential role in preserving genome stability through maintaining balanced dNTP pools. Independently of its dNTPase activity, SAMHD1 is required for resection of stalled, gapped, or reversed DNA replication forks by stimulating the exonuclease activity of MRE11. SAMHD1-deficient cells show increased dNTPs and genome instability, and an increased accumulation of G1-positive cells. These cells also accumulate single-strand DNA fragments in the cytosol, leading to the induction of pro-inflammatory type 1 interferons through the cGAS-STING pathway. Mutations in the SAMHD1 are found in many cancers, as well as the severe congenital inflammatory disease Aicardi-Goutieres syndrome.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant human SAMHD1 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Clone W19081C is not recommended for immunocytochemistry applications due to non-specific staining.
- Additional Product Notes
-
Clone W19081C can be used with Cyto-Fast™ Fix/Perm Buffer or True-Nuclear™ Transcription Factor Buffer. However, True-Phos™ Perm Buffer is recommended for optimal staining results.
- RRID
-
AB_3662343 (BioLegend Cat. No. 941003)
AB_3662343 (BioLegend Cat. No. 941004)
Antigen Details
- Structure
- SAMHD1 is a 626 amino acid protein with a predicted molecular weight of 72 kD.
- Distribution
-
Ubiquitously expressed/Nucleus
- Function
- Innate immune response, DNA replication and repair
- Cell Type
- Dendritic cells, Monocytes
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Innate Immunity
- Molecular Family
- Enzymes and Regulators
- Antigen References
-
- Laguette N, et al. 2011. Nature. 474:654.
- Gene ID
- 25939 View all products for this Gene ID
- UniProt
- View information about SAMHD1 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All SAMHD1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-SAMHD1 | W19081C | WB,IP,ICFC |
| PE anti-SAMHD1 | W19081C | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-SAMHD1
Whole cell extracts (15 µg total protein) from the indicated... 
Whole cell extracts (250 µg total protein) prepared from Dau... 
Human peripheral blood mononuclear cells were fixed and perm... 
THP1 cells were fixed and permeabilized using True-Phos™ Per... -
PE anti-SAMHD1
Human peripheral blood mononuclear cells were surface staine... 
THP1 cells were fixed and permeabilized using True-Phos™ Per...
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