- Clone
- HI30 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- IV N816
- Other Names
- LCA, T200
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
304027 | 25 tests | 128€ | ||||
304028 | 100 tests | 272€ |
CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The CD45 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
SB - Community Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, Western blotting3, and spatial biology (IBEX)16,17.
It was found that the HI30 clone and the 2D1 clone can cross block each other's binding. - Additional Product Notes
-
For the use of this antibody in spatial biology (SB), we have partnered with Bruker Spatial Biology Biosciences for demonstration of this antibody on their next-generation ChipCytometry instrument called the CellScape™. The CellScape platform is an end-to-end solution for highly multiplexed spatial omics. Combining an advanced, purpose-built imaging system with easy-to-use fluidics for walk-away automation, the CellScape system will accelerate your exploration into the rapidly evolving field of spatial biology. More information on the the Bruker Spatial Biology CellScape and a complete list of our antibodies that have been demonstrated on the instrument can be found here.
- Application References
-
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Kishihara K, et al. 1993. Cell 74:143.
- Esser M, et al. 2001. J. Virol. 75:6173. (WB)
- Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
- Nagano M, et al. 2007. Blood 110:151.
- Jiang Q, et al. 2008. Blood 112:2858. PubMed
- Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
- Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
- Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
- Lee J, et al. 2015. J Exp Med. 212:385. PubMed
- Breton G, et al. 2015. J Exp Med. 212:401. PubMed
- Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
- Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
-
AB_893338 (BioLegend Cat. No. 304027)
AB_893338 (BioLegend Cat. No. 304028)
Antigen Details
- Structure
- Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
- Distribution
-
Hematopoietic cells, not expressed in circulating erythrocytes or platelets
- Function
- TCR and BCR mediated activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Cell Type
- Hematopoietic stem and progenitors, Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
- Thomas M. 1989. Annu. Rev. Immunol. 7:339.
- Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45 on UniProt.org
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
-
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
- If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?
-
It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.
- Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?
-
Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.
- Are other fluorophores compatible with IBEX?
-
Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.
- The same antibody works in one tissue type but not another. What is happening?
-
Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.
- How can I be sure the staining I’m seeing in my tissue is real?
-
In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.
Other Formats
View All CD45 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD45
Human peripheral blood lymphocytes stained with HI30 APC -
Biotin anti-human CD45
Human peripheral blood lymphocytes stained with biotinylated... -
FITC anti-human CD45
Human peripheral blood lymphocytes, monocytes and granulocyt... -
PE anti-human CD45
Human peripheral blood lymphocytes stained with HI30 PE PE anti-human CD45 Antibody Human peripheral blood was stai... -
PE/Cyanine5 anti-human CD45
Human peripheral blood lymphocytes stained with HI30 PE/Cyan... -
Purified anti-human CD45
Human peripheral blood lymphocytes were stained with purifie... Preparation and staining of formalin fixed paraffin-embedded... -
APC/Cyanine7 anti-human CD45
Human peripheral blood lymphocytes stained with CD45 (clone ... -
PE/Cyanine7 anti-human CD45
Human peripheral blood lymphocytes stained with HI30 PE/Cyan... -
Alexa Fluor® 488 anti-human CD45
Human peripheral blood lymphocytes stained with HI30 Alexa F... -
Alexa Fluor® 647 anti-human CD45
Human peripheral blood lymphocytes stained with HI30 Alexa F... -
Pacific Blue™ anti-human CD45
Human peripheral blood lymphocytes stained with HI30 Pacific... -
Alexa Fluor® 700 anti-human CD45
Human peripheral blood lymphocytes stained with HI30 Alexa F... -
PerCP anti-human CD45
Human peripheral blood lymphocytes stained with HI30 PerCP -
PerCP/Cyanine5.5 anti-human CD45
Human peripheral blood lymphocytes were stained with HI30 Pe... Multiplexed IHC staining of PerCP/Cyanine5.5 anti-CD45 (clon... -
Brilliant Violet 421™ anti-human CD45
Human peripheral blood lymphocytes were stained with CD45 (c... -
Brilliant Violet 570™ anti-human CD45
RBC-lysed human peripheral blood cells were stained with CD4... -
Brilliant Violet 510™ anti-human CD45
Human peripheral blood lymphocytes were stained with CD45 (c... -
Brilliant Violet 605™ anti-human CD45
Human peripheral blood lymphocytes were stained with CD45 (c... -
Brilliant Violet 650™ anti-human CD45
Human peripheral blood lymphocytes were stained with CD45 (c... -
Purified anti-human CD45 (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30). Data provid... -
Brilliant Violet 785™ anti-human CD45
Human peripheral blood lymphocytes were stained with CD45 (c... -
Brilliant Violet 711™ anti-human CD45
Human peripheral blood lymphocytes were stained with CD45 (c... -
PE/Dazzle™ 594 anti-human CD45
Human peripheral blood lymphocytes were stained with CD45 (c... -
Alexa Fluor® 594 anti-human CD45
Human paraffin-embedded tonsil tissue slices were prepared w... Confocal image of human liver sample acquired using the IBEX... -
APC/Fire™ 750 anti-human CD45
Human peripheral blood lymphocytes were stained with CD45 (c... -
Pacific Blue™ anti-human CD45
Typical results from human peripheral blood lymphocytes stai... -
APC anti-human CD45
Typical results from human peripheral blood lymphocytes stai... -
PE/Cyanine7 anti-human CD45
Typical results from human peripheral blood lymphocytes stai... -
PE/Dazzle™ 594 anti-human CD45
Typical results from human peripheral blood lymphocytes sta... -
TotalSeq™-A0391 anti-human CD45
-
TotalSeq™-B0391 anti-human CD45
-
TotalSeq™-C0391 anti-human CD45
-
PerCP/Cyanine5.5 anti-human CD45
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 750 anti-human CD45
Typical results from human peripheral blood lymphocytes stai... -
PE/Fire™ 640 anti-human CD45
Human peripheral blood lymphocytes were stained with CD45 (c... -
APC/Fire™ 810 anti-human CD45
Human peripheral blood lymphocytes were stained with CD45 (c... -
Spark YG™ 570 anti-human CD45
Human paraffin-embedded tonsil tissue slices were prepared w... -
PE/Fire™ 700 anti-human CD45
Human peripheral blood lymphocytes were stained with anti-hu... -
FITC anti-human CD45
Typical results from human peripheral blood lymphocytes stai... -
PerCP anti-human CD45
Typical results from human peripheral blood lymphocytes stai... -
Alexa Fluor® 660 anti-human CD45 Antibody
Human peripheral blood lymphocytes were stained with CD45 (c... -
Spark Violet™ 538 anti-human CD45
Human peripheral blood lymphocytes were stained with CD45 (c... -
Spark YG™ 593 anti-human CD45
Human peripheral blood lymphocytes were stained with CD45 (c... -
GMP APC/Fire™ 750 anti-human CD45
Typical results from human peripheral blood lymphocytes stai... -
Spark Violet™ 538 anti-human CD45
Typical results from human peripheral blood lymphocytes stai... -
GMP APC anti-human CD45
Typical results from human peripheral blood lymphocytes stai... -
Spark UV™ 387 anti-human CD45
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP Pacific Blue™ anti-human CD45
Typical results from human peripheral blood lymphocytes stai... -
GMP PerCP anti-human CD45
Typical results from human peripheral blood lymphocytes stai... -
GMP FITC anti-human CD45
Typical results from human peripheral blood lymphocytes stai... -
PE anti-human CD45
Typical results from human peripheral blood lymphocytes stai... -
GMP PE/Dazzle™ 594 anti-human CD45
Typical results from human peripheral blood lymphocytes stai... -
GMP PerCP/Cyanine5.5 anti-human CD45
Typical results from human peripheral blood lymphocytes stai... -
Spark Blue™ 515 anti-human CD45
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-D0391 anti-human CD45
-
GMP PE/Cyanine7 anti-human CD45
Typical results from human peripheral blood lymphocytes stai... -
Spark Violet™ 500 anti-human CD45
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PE anti-human CD45
Typical results from human peripheral blood lymphocytes stai... -
PE/Fire™ 810 anti-human CD45
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark PLUS UV395™ anti-human CD45
Human peripheral blood lymphocytes were stained with anti-hu... Human peripheral blood cells were stained with anti-human CD... -
Spark NIR™ 685 anti-human CD45
Human peripheral blood leukocytes were stained with anti-hum... -
Spark Violet™ 423 anti-human CD45
Human peripheral blood leukocytes were stained with anti-hum...
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