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PerCP/Cyanine5.5 anti-mouse CD43 Antibody

Pricing & Availability
Clone
S11 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-48
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
S11_PerCPCyanine5dot5_CD43_Antibody_032619.png
C57BL/6 mouse splenocytes were stained with anti-mouse CD43 (clone S11) PerCP/Cyanine5.5 (filled histogram) or rat IgG2b Percp/Cyanine5.5 isotype control (open histogram).
  • S11_PerCPCyanine5dot5_CD43_Antibody_032619.png
    C57BL/6 mouse splenocytes were stained with anti-mouse CD43 (clone S11) PerCP/Cyanine5.5 (filled histogram) or rat IgG2b Percp/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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143219 25 µg 118€
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143220 100 µg 216€
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Description

CD43, also known as Leukosialin and Ly48, is a 125 kD sialoprotein (glycosylated protein) expressed from 1.2 kBase mRNA in bone marrow-derived cells. This occurs early in development. Cells expressing CD43 include γ/δ T cells, macrophages, mature B cells, and dendritic cells. CD43 functions as an anti-adhesive surface molecule, promoted by antibody cross-linking, that releases the trailing edge of the cell during locomotion to allow movement of the cell body towards the lamellipodia. The intracellular distribution of CD43 is determined by binding to moesin, an intracellular membrane protein, which is in-turn bound in some manner to the actin cytoskeleton. Defects with CD43 function and expression retard cellular locomotion, resulting in a wide range of immune disorders. Wiscott-Alderich syndrome, and the varying degrees of its severity, is related to the dysregulation of CD43 expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse plasmacytoma cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting3. The S11 antibody reacts with pan-CD43.

Application References
  1. Gaspari AA, et al. 1993. J. Invest. Dermatol. 100:247. (FC)
  2. Merzaban JS, et al. 2005. J. Immunol. 174:4051. (FC)
  3. Baecher-Allan CM, et al. 1993. Immunogenetics. 37:183. (WB)
Product Citations
  1. Kobayashi M, et al. 2023. Cell Rep. 112239:42. PubMed
RRID
AB_2800666 (BioLegend Cat. No. 143219)
AB_2800666 (BioLegend Cat. No. 143220)

Antigen Details

Structure
Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites; 115 kD and 130 kD glycoforms with different glycosylation and sialylation.
Distribution

Gamma/delta T cells, macrophages, mature B cells, and dendritic cells.

Function
Plays dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors.
Interaction
CD43 functions as an anti-adhesive surface molecule, promoted by antibody cross-linking, that releases the trailing edge of the cell during locomotion to allow movement of the cell body towards the lamellipodia.
Ligand/Receptor
Moesin, CD54, E-selectin, Siglec-1.
Cell Type
B cells, Dendritic cells, Macrophages, T cells
Biology Area
Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Van den Berg TK, et al. 2001. J. Immunol. 166:3637.
2. Moore T, et al. 1994. J. Immunol. 153:4978.
3. Onami TM, et al. 2002. J. Immunol. 168:6022.
4. Tong J, et al. 2004. J. Exp. Med. 199:1277.
5. Jones AT, et al. 1994. J. Immunol. 153:3426.
6. Matsumoto M, et al. 2005. J. Immunol. 175:8042.

Gene ID
20737 View all products for this Gene ID
UniProt
View information about CD43 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 06/09/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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