Propidium Iodide Solution

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Regulatory Status
RUO
Other Names
PI
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421301 2 mL 44€
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Description

Propidium iodide (PI) is a fluorescent dye that binds to DNA. When excited by 488nm laser light, it can be detected with in the PE/Texas Red® channel with a bandpass filter 610/10. It is commonly used in evaluation of cell viability or DNA content in cell cycle analysis by flow cytometry.

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide.
Concentration
0.5 mg/ml
Storage & Handling
The solution should be stored undiluted between 2°C and 8°C, and protect from light. Caution: This solution contains hazardous material; handle with care.
Application

FC - Quality tested

Recommended Usage

The suggested use of this solution for viability staining is 10 µl per million cells in 0.5 ml/test, and incubate for 15 minutes at 4 °C before analysis. For Cell Cycle analysis, please see our Propidium Iodide Cell Cycle Staining Protocol. Caution: This solution is toxigenic and mutagenic; handle with care.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Propidium Iodide Solution can be used in evaluation of apoptosis, cell viability and cell cycle analysis by flow cytometry.

Application References
  1. Vermes I, et al.1995. J. Immunol. Methods 184:39.
  2. Darzynkiewicz Z, et al. 1992. Cytometry 13(8):795.
  3. Douglas RS, et al. 1995. J. Immunol. Methods 188:219.
  4. Sakimoto I, et al. 2006. Cancer Research 66:2287.PubMed
  5. Liu H, et al. 2012. Int J Biochem Cell Biol. 45:408. PubMed
  6. Juel HB, et al. 2013. PLoS One. 8:64619. PubMed
  7. Ren Q, et al. 2013. PLoS One. 8:74732. PubMed
  8. Zhao W, et al. 2013. Clin Immunol. 149:119. PubMed
  9. Beggs KM, et al. 2014. Toxicol Sci. 137:91. PubMed
  10. Ewald SE, et al. 2014. Infect Immun. 82:460. PubMed
  11. Tsou WI, et al. 2014. J Biol Chem. 289:25750. PubMed
  12. Kong S, et al. 2014. J Immunol. 193:5515. PubMed
Product Citations
  1. Glynn RA, et al. 2022. J Immunol. 208:371. PubMed
  2. Marques de Menezes EG, et al. 2022. MBio. 13:e0300521. PubMed
  3. Das Gupta D, et al. 2022. Cell Death Differ. 29:2163. PubMed
  4. Qin L, et al. 2022. Sci Adv. 8:eabn3774. PubMed
  5. Cheng Y, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:964. PubMed
  6. Choi UY, et al. 2022. Cell Rep. 40:111234. PubMed
  7. Park J, et al. 2022. Animals (Basel). 12: . PubMed
  8. Papakonstantinou A, et al. 2022. Int J Mol Sci. 24: . PubMed
  9. Blal K, et al. 2023. Cancers (Basel). 15: . PubMed
  10. Xiong E, et al. 2023. Front Immunol. 13:1083119. PubMed
  11. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  12. Bhardwaj M, et al. 2023. J Clin Invest. :. PubMed
  13. Hochrein SM, et al. 2022. Cell Metab. 34:516. PubMed
  14. Wenes M, et al. 2022. Cell Metab. 34:731. PubMed
  15. Liu H, et al. 2012. Int J Biochem Cell Biol. 45:408. PubMed
  16. Ewald S, et al. 2014. Infect Immun . 82:460. PubMed
  17. Guo H, et al. 2016. Sci Rep. 6:37559. PubMed
  18. Smith A, et al. 2017. Sci Rep. 7:41872. PubMed
  19. Li W, et al. 2019. Aging (Albany NY). 0.99375. PubMed
  20. Evans SJ, et al. 2022. Toxicol In Vitro. 83:105415. PubMed
  21. Hagege A, et al. 2022. Cell Rep Med. 3:100659. PubMed
  22. Tintelnot J, et al. 2023. Nature. 615:168. PubMed
  23. Ohuchi K, et al. 2023. Biomed Rep. 18:28. PubMed
  24. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  25. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  26. Quintela M, et al. 2023. Br J Cancer. . PubMed
  27. Sohrabi N, et al. 2023. Iran J Public Health. 52:633. PubMed
  28. Papsdorf K, et al. 2023. Nat Cell Biol. 25:672. PubMed
  29. Schwartz PB, et al. 2023. PLoS Genet. 19:e1010770. PubMed
  30. Wyrobnik I, et al. 2023. Oncoimmunology. 12:2219164. PubMed
  31. Lindqvist B, et al. 2022. PLoS Pathog. 18:e1010555. PubMed
  32. Chan J, et al. 2017. Oncogene. 10.1038/onc.2017.319. PubMed
  33. Uroda T, et al. 2019. Mol Cell. 75:982. PubMed
  34. Hamel Y, et al. 2021. Cell Rep Med. 2:100370. PubMed
  35. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  36. Leeth C, et al. 2021. Animals (Basel). 11:. PubMed
  37. Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
  38. McNeal AS, et al. 2021. Elife. 10:. PubMed
  39. Neumann CKA, et al. 2020. Mol Metab. 34:136. PubMed
  40. Yoshioka H, et al. 2021. Front Cell Dev Biol. 9:621045. PubMed
  41. Yang Y, et al. 2021. Metabolism. 123:154863. PubMed
  42. Sun X, et al. 2022. JCI Insight. 7: . PubMed
  43. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  44. Ren Q, et al. 2013. PLoS One. 8:74732. PubMed
  45. He Y, et al. 2020. Nat Commun. 11:1996. PubMed
  46. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  47. Zhang W, et al. 2021. J Clin Invest. 131:. PubMed
  48. Ambrosi TH, et al. 2021. Elife. 10: . PubMed
  49. Schrier S, et al. 2016. Sci Rep. 6:34942. PubMed
  50. O'Neill K, et al. 2016. Genes Dev. 30: 973-988. PubMed
  51. Sapanidou V, et al. 2015. Theriogenology. . PubMed
  52. Kong S, et al. 2014. J Immunol. 193:5515. PubMed
  53. Zeng H, et al. 2018. Cancer Cell. 34:56:00. PubMed
  54. Lei W, et al. 2020. Sci Rep. 10:7004. PubMed
  55. Wang Y, et al. 2019. Cell. 179:1144. PubMed
  56. Zhang M, et al. 2017. Oncol Lett. 3.069444444. PubMed
  57. Zou PA, et al. 2021. Oncol Lett. 22:648. PubMed
  58. Masuda T, et al. 2022. iScience. 25:104781. PubMed
  59. Feng L, et al. 2016. J Cell Sci. 129: 2202 - 2212. PubMed
  60. Lange N, et al. 2016. PLoS One. 11: 0160843. PubMed
  61. Jordan PM, et al. 2020. Cell Rep. 33:108247. PubMed
  62. Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
  63. Liu L, et al. 2019. Cell Prolif. :e12718. PubMed
  64. Reighard SD, et al. 2020. Cell Rep Med. :1. PubMed
  65. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  66. Xu B, et al. 2016. Leukemia. 10.1038/leu.2016.297. PubMed
  67. Liu Y, et al. 2017. Acta Biomater. 10.1016/j.actbio.2017.08.021. PubMed
  68. Yang M, et al. 2021. Med Sci Monit. 27:e930083. PubMed
  69. Domenis R, et al. 2017. PLoS One. 10.1371/journal.pone.0169932. PubMed
  70. Walter F, et al. 2020. PLoS One. 15:e0239369. PubMed
  71. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  72. Pang K, et al. 2022. Cell Death Dis. 13:169. PubMed
  73. Tang X, et al. 2021. Transl Cancer Res. 10:2120. PubMed
  74. Zhang X, et al. 2020. Eur J Med Chem. 112186:192. PubMed
  75. Mensen A, et al. 2015. Biol Blood Marrow Transplant. Available online 10 August 2015. PubMed
  76. Zhao W, et al. 2013. Clin Immunol. 149:119. PubMed
  77. Liu H, et al. 2020. Cell Death Dis. 0.767361111. PubMed
  78. Li L, et al. 2018. Oncol Lett. 4.184722222. PubMed
  79. Jung E, et al. 2017. J Neurosci. 37:6837. PubMed
  80. Basso-Ricci L, et al. 2017. Cytometry A. 10.1002/cyto.a.23148. PubMed
  81. Shanahan MT, et al. 2021. Am J Physiol Gastrointest Liver Physiol. 321:G668. PubMed
  82. Wang J, et al. 2022. J Clin Invest. :. PubMed
  83. Trudu M, et al. 2022. Front Oncol. 12:968208. PubMed
  84. Li Y, et al. 2016. Int J Lower Extrem Wounds. 10.1177/1534734616649485. PubMed
  85. Hu D, et al. 2020. Proc Natl Acad Sci U S A. 117:30639. PubMed
  86. Li Q, et al. 2020. Acta Biochim Biophys Sin (Shanghai). 52:1131. PubMed
  87. Moribe F, et al. 2021. PLoS One. 16:e0237554. PubMed
  88. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  89. Xue G, et al. 2021. Nat Biomed Eng. 5:1306. PubMed
  90. Lasrado N, et al. 2022. iScience. 25:103865. PubMed
  91. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  92. Perl AL, et al. 2019. J Biol Chem. 294:17043. PubMed
  93. Diaz Osterman CJ, et al. 2019. Elife. 8:e47327. PubMed
  94. Hosokawa T, et al. 2017. J Immunol. 10.4049/jimmunol.1700157. PubMed
  95. El-Hafeez A, et al. 2017. Cytotechnology. 10.1007/s10616-017-0124-1. PubMed
  96. Tsou W, et al. 2014. J Biol Chem. 289:25750. PubMed
  97. Oubaha M, et al. 2016. Sci Transl Med. 8: 362ra144. PubMed
  98. Polk A, et al. 2016. Clin Cancer Res. 22(24):6118-6128. PubMed
  99. Pal P, et al. 2021. J Med Chem. 64:14230. PubMed
  100. Hedemann N, et al. 2021. Cancers (Basel). 13:. PubMed
  101. Xie B, et al. 2021. J Immunol. 206:1515. PubMed
  102. Harro CM, et al. 2020. J Clin Invest. . PubMed
  103. Imura Y, et al. 2020. JCI Insight. :5. PubMed
  104. Suzuki S, et al. 2021. STAR Protocols. 2:100683. PubMed
  105. Vickman R, et al. 2016. Mol Cancer Res. 14: 776 - 786. PubMed
  106. El-Hafeez A, et al. 2017. Cytotechnology. 10.1007/s10616-017-0116-1. PubMed
  107. Xie M, et al. 2017. BMC Cancer . 10.1186/s12885-017-3291-8. PubMed
  108. Matsubara T, et al. 2022. J Exp Med. 219:. PubMed
  109. M H, et al. 2016. Cell Transplant. 25: 811-27. PubMed
  110. Woo MS, et al. 2021. J Exp Med. :218. PubMed
  111. Huang K, et al. 2018. Biol Open. 7:bio029470. PubMed
  112. Wang H, et al. 2019. Nat Commun. 4.388194444. PubMed
  113. Viereckl MJ, et al. 2022. Biomolecules. 12:. PubMed
  114. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  115. Hughes K, et al. 2017. Open Bio. 10.1098/rsob.160155. PubMed
  116. Resch U, et al. 2016. MBio. 7: e00207-16. PubMed
  117. Bao W, et al. 2016. Sci Rep. 6:22579. PubMed
  118. Liu X, et al. 2019. Mol Cancer Ther. 18:706. PubMed
  119. Yang X, et al. 2017. J Control Release. 10.1016/j.jconrel.2017.07.014. PubMed
  120. Ghura H, et al. 2021. Neoplasia. 23:837. PubMed
  121. Beggs K, et al. 2014. Toxicol Sci. 137:91. PubMed
  122. Wang C, et al. 2017. Drug Deliv. 10.1080/10717544.2017.1293195. PubMed
  123. Nilsri N, et al. 2021. Sci Rep. 5255:11. PubMed
  124. Bjørnstad R, et al. 2019. Mol Cancer Ther. 1.14375. PubMed
  125. Widodo N, et al. 2022. F1000Res. 11:169. PubMed
  126. Tu Z, et al. 2022. Cell Rep. 40:111072. PubMed
  127. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  128. He Y, et al. 2020. J Hematol Oncol. 0.607638889. PubMed
  129. Goel S, et al. 2021. Nat Commun. 12:5325. PubMed
  130. Szeto C, et al. 2022. Nat Commun. 13:4951. PubMed
  131. Namas R, et al. 2016. Lupus Sci Med. 3: e000148. PubMed
  132. Chen Y, et al. 2016. J Biol Chem. 291: 13216 - 13228. PubMed
  133. Deng BB, et al. 2020. Cell Biol Int. 44:1890. PubMed
  134. Siddhartha V, et al. 2017. Artif Cells Nanomed Biotechnol . 10.1080/21691401.2017.1313267. PubMed
  135. Viseshakul N, et al. 2017. Parasitol Res. 10.1007/s00436-017-5589-5. PubMed
  136. Chmielewski M and Abken H 2017. Cell Rep.. 10.1016/j.celrep.2017.11.063. PubMed
  137. Sakimoto I, et al. 2006. Cancer Res. 66:2287. PubMed
  138. Vulin M, et al. 2022. Oncogene. :. PubMed
  139. Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed
  140. Mahmoudi M, et al. 2016. Sci Rep. 6:26960. PubMed
  141. Juel H, et al. 2013. PLoS One. 8:64619. PubMed
  142. Popa S, et al. 2020. Wellcome Open Res. 0.249305556. PubMed

Antigen Details

Biology Area
Cell Biology, Cell Cycle/DNA Replication, Neuroscience
Gene ID
NA

Related FAQs

Why is washing not recommended after the addition 7-AAD or PI addition when assessing viability?

These dyes bind in equilibrium with DNA. Therefore, external dye concentration must be maintained during analysis and the dye should not be washed out.

Go To Top Version: 2    Revision Date: 10/08/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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