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Purified anti-ALDH2 Antibody

Pricing & Availability
Clone
8D4-D6-D11 (See other available formats)
Regulatory Status
RUO
Other Names
ALDH2, Aldehyde Dehydrogenase 2 Family (Mitochondrial)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
8D4-D6-D11_PURE_ALDH2_Antibody_1_062419
Western blot of purified anti-ALDH2 antibody (clone 8D4-D6-D11). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain lysate; Lane 3: 30 µg of mouse brain lysate; Lane 4: 10 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 8D4-D6-D11_PURE_ALDH2_Antibody_1_062419
    Western blot of purified anti-ALDH2 antibody (clone 8D4-D6-D11). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain lysate; Lane 3: 30 µg of mouse brain lysate; Lane 4: 10 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 8D4-D6-D11_PURE_ALDH2_Antibody_2_062419
    IHC staining of purified anti-ALDH2 antibody (clone 8D4-D6-D11) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 8D4-D6-D11_PURE_ALDH2_Antibody_3_062419
    IHC staining of purified anti-ALDH2 antibody (clone 8D4-D6-D11) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 8D4-D6-D11_PURE_ALDH2_Antibody_4_062419
    IHC staining of purified anti-ALDH2 antibody (clone 8D4-D6-D11) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Check Availability Save
866801 25 µg 85€
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866802 100 µg 221€
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Description

ALDH2 is a member of the aldehyde dehydrogenase family. In the human brain, ALDH2 is the major aldehyde metabolizing enzyme that is essential for the metabolism and detoxification of endogenous and exogenous aldehyde substrates. In line with this, recent studies have reported that ALDH2 level is dysregulated in the brains of patients with Parkinson’s and Alzheimer's diseases, suggesting toxic aldehydes as a contributing factor to neurodegenerative diseases and a potential therapeutic target.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant human ALDH2 fragments expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810791 (BioLegend Cat. No. 866801)
AB_2810791 (BioLegend Cat. No. 866802)

Antigen Details

Structure
ALDH2 is a 517 amino acid protein with predicted and observed molecular mass of ~ 56 kD.
Distribution

Tissue Distribution: Mainly expressed in adrenal gland, lung, kidney, and brain
Cellular Distribution: Mitochondrion and cytosol

Function
Aldehyde metabolizing enzyme that is essential for the metabolism and detoxification of endogenous and exogenous aldehyde substrates.
Interaction
Forms homo-tetramers
Cell Type
Astrocytes, Microglia, Neurons, Oligodendrocytes
Biology Area
Mitochondrial Function, Neurodegeneration, Neuroinflammation, Neuroscience
Molecular Family
Aldehyde Dehydrogenases, Mitochondrial Markers
Antigen References
  1. Chen CH, et al. 2016. Acta. Neurol. Taiwan. 25(4):111-123.
  2. Chauhan G, Debette S. 2016. Curr. Cardiol. Rep. 18(12):124.
  3. Grünblatt E, Riederer P. 2016. J. Neural Transm. (Vienna). 123(2):83-90.
Gene ID
217 View all products for this Gene ID 11669 View all products for this Gene ID 29539 View all products for this Gene ID
UniProt
View information about ALDH2 on UniProt.org
Go To Top Version: 1    Revision Date: 06/24/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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