- Clone
- QA20A98 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- β-tubulin, tubulin beta chain (TUBB), tubulin beta-5 chain, TUBB5, TUBB1, CSCSC1, Tubulin Beta class I, Epididymid Secretory Sperm Binding Protein, M40
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
607151 | 25 µg | 88€ | ||||
607152 | 100 µg | 300€ |
Eukaryotic microtubules are tubular polymers of tubulin that are a major component of the cytoskeleton. They play an essential role in diverse cellular functions, including motility, mitosis, and trafficking, and are also critical for normal development. β- and α-tubulins comprise the two core protein families that heterodimerize and assemble into each microtubule filament. There are multiple β-tubulin isotypes, with each showing unique tumoral and tissue-specific distribution.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Amino acids 412-430 of purified bovine brain tubulin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P, ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
For ICC, staining was compatible with fixation with 100% ice-cold methanol, or fixation with 4% paraformaldehyde followed by permeabilization with 100% ice-cold methanol or 0.5% Triton-X.
This antibody is not designed to be specific against any particular isoform of β-tubulin. - RRID
-
AB_2924621 (BioLegend Cat. No. 607151)
AB_2924621 (BioLegend Cat. No. 607152)
Antigen Details
- Structure
- β-tubulin is a 444 amino acid protein with a predicted molecular weight of 49.7 kD.
- Distribution
-
β-tubulin is ubiquitously expressed in most cell types
- Function
- β-tubulin is a GTPase whose polymerization is regulated by GTP hydrolysis
- Interaction
- β-tubulin homodimerizes with α-tubulin to form microtubules
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microtubules
- Antigen References
-
- Lopes D, Maiato H. 2020. Cells. 9:2356.
- Wade RH. 2007. Methods Mol. Med. 137:1-16.
- Gene ID
- 203068 View all products for this Gene ID
- UniProt
- View information about beta-Tubulin on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All β-Tubulin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-β-Tubulin Recombinant Antibody | QA20A98 | WB,IHC-P,ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-β-Tubulin Recombinant Antibody
Whole cell extracts (15 µg total protein) from the indicated... IHC staining using purified anti-β-Tubulin recombinant (clon... ICC staining of purified anti-β-Tubulin recombinant (c...
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