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Purified anti-Caspase-2L Antibody

Pricing & Availability
Clone
W16017A (See other available formats)
Regulatory Status
RUO
Other Names
CASP-2, Neural precursor cell expressed developmentally down-regulated protein 2 (NEDD-2), Protease ICH-1,
Isotype
Rat IgG2a, κ
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Product Citations
publications
W16017A_PURE_Caspase2L_Antibody_051017
Total cell lysates (15 µg protein) from EL-4, NTERA-2 and Jurkat were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 2 µg/mL purified anti-Caspase-2L (clone W16017A) antibody (upper) or loading control anti-GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-rat-IgG secondary antibody conjugated to HRP for anti-Caspase-2L antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for anti-GAPDH antibody. Lane M: molecular weight ladder.
  • W16017A_PURE_Caspase2L_Antibody_051017
    Total cell lysates (15 µg protein) from EL-4, NTERA-2 and Jurkat were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 2 µg/mL purified anti-Caspase-2L (clone W16017A) antibody (upper) or loading control anti-GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-rat-IgG secondary antibody conjugated to HRP for anti-Caspase-2L antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for anti-GAPDH antibody. Lane M: molecular weight ladder.
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697002 100 µg 231€
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Description

Caspase-2 is an evolutionarily conserved member of the caspase family. Caspases are cycteine-rich proteases, known to be activated in response to apoptotic stimuli including DNA damage, ER stress, oxidative stress, and heat shock. Activated caspases hydrolyze structural proteins in the cells that in turn results in apoptosis. Upon activation, Caspase-2 leads to proteolytic cleavage of the pro-apoptotic protein Bid and facilitates permeabilization of mitochondrial outer membrane. In addition to its function in cell death, Caspase-2 has been shown to participate in affecting genomic stability, aging, autophagy, and tumor suppression. Reduced Caspase-2 expression has been found to associate with Burkitt's lymphoma and correlate with poor prognosis in myeloid leukemia.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial human caspase-2 recombinant protein (203-452 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

As per the Human Protein Atlas RNA level dataset, NTERA-2 is expected to have a low level of caspase-2L expression.

This antibody only recognizes the pro-form of caspase-2L.

RRID
AB_2687108 (BioLegend Cat. No. 697002)

Antigen Details

Structure
452 amino acids with a predicted molecular weight of 50.7 kD. Cleaved into 3 subunit, p18, p13, and p12, upon activation.
Distribution

Cytoplasm, mitochondrion, membrane, nucleus.

Function
Caspase-2 is a proteolytic enzyme known to activate apoptotic pathways and play non-apoptotic roles affecting genomic stability, aging, autophagy, and tumor suppression.
Interaction
Forms a heterotetramer that consists of p18 and p12 subunits. Interacts with LRDD and NOL3.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology
Antigen References

1. Shalini S, et al. 2016. Cell Death Differ. 23:1727.
2. Olsson M, et al. 2015. Oncogene. 34:1877.
3. Shalini S, et al. 2015. Oncogene. 34:4995.
4. Puccini J, Dorstyn L, Kumar S, et al. 2013. Cell Death Differ. 20(9):1133.
5. Fava LL, et al. 2012. J. Cell Sci. 125:5911.
6. Bouchier-Hayes L. 2010. J. Cell. Mol. Med. 14:1212.

Gene ID
835 View all products for this Gene ID
UniProt
View information about Caspase-2L on UniProt.org
Go To Top Version: 2    Revision Date: 03/23/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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