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Purified anti-COX IV Antibody

Pricing & Availability
Clone
4D11-B3-E8 (See other available formats)
Regulatory Status
RUO
Other Names
COX4I1, Cytochrome C Oxidase Subunit 4I1, Cytochrome C Oxidase Subunit 4 Isoform 1, Mitochondrial, Cytochrome C Oxidase Subunit IV Isoform 1, Cytochrome C Oxidase Polypeptide IV, COX IV-1, COX4
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
4D11-B3-E8_PURE_COX-IV_Antibody_1_081020.png
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-COX IV antibody (clone 4D11-B3-E8) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • 4D11-B3-E8_PURE_COX-IV_Antibody_1_081020.png
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-COX IV antibody (clone 4D11-B3-E8) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • 4D11-B3-E8_PURE_COX-IV_Antibody_2_081020.png
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg of purified mouse IgG1, κ isotype ctrl antibody (Cat. No. 401402) or purified anti-COX IV antibody (clone 4D11-B3-E8). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 4D11-B3-E8. Lane M: Molecular weight marker.
  • 4D11-B3-E8_PURE_COX-IV_Antibody_3_081020.png
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with methanol for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 5.0 µg/mL of purified anti-COX IV antibody (panel A) followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) at 2.0 µg/mL. Alexa Fluor® 647 anti-Cytochrome c antibody (Cat. No. 612310) was used at 5.0 µg/mL to co-stain the mitochondria and is presented in green pseudocolor (panel B). The merged image is shown in panel C. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
Cat # Size Price Quantity Check Availability Save
937801 25 µg 81€
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937802 100 µg 203€
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Description

COX IV is a subunit of cytochrome C oxidase (COX), a critical component of the mitochondrial respiratory pathway. COX is an enzyme comprised of multiple subunits which work to remove electrons from cytochrome C and transfer them to molecular oxygen, this action also promotes proton pumping from the mitochondrion matrix to the intermembrane space to maintain electrochemical stability. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic human COXIV peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1 µg/mL. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC using 4% PFA-fixed HeLa cells permeabilized with either methanol or Triton X-100. While staining was observed with both methods, methanol permeabilization resulted in stronger staining.

RRID
AB_2876756 (BioLegend Cat. No. 937801)
AB_2876756 (BioLegend Cat. No. 937802)

Antigen Details

Structure
COX IV is a 169 amino acid protein with a predicted molecular weight of 19 kD.
Distribution

Mitochondria

Biology Area
Cell Biology, Mitochondrial Function
Molecular Family
Enzymes and Regulators
Antigen References
  1. Capaldi RA. 1990. Annu Rev Biochem. 59:569.
  2. Sharma V, et al. 2015. Proc Natl Acad Sci U S A. 112:2040-5.
  3. Li Y, et al. 2006. J Bioenerg Biomembr. 38:283-91.
Gene ID
1327 View all products for this Gene ID
UniProt
View information about COX IV on UniProt.org
Go To Top Version: 1    Revision Date: 08/10/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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