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Purified anti-EGFR Phospho (Tyr1045) Antibody

Pricing & Availability
Clone
Poly23A05 (See other available formats)
Regulatory Status
RUO
Other Names
Epidermal Growth Factor Receptor, Receptor Tyrosine-Protein Kinase ErbB-1, Erb-B2 Receptor Tyrosine Kinase 1, ERBB1, NISBD2, PIG61, MENA
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly23A05_PURE_EGFR_Antibody_1_120524
Whole cell extracts (15 µg total protein) from A-431 cells either untreated (-) or treated (+) with EGF (Cat. No. 585506) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL of Purified anti-EGFR Phospho (Tyr1045) (clone Poly23A05) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP Donkey anti-rabbit IgG (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Total EGFR loading was confirmed using Purified anti-EGFR (clone A19002A) (Cat. No. 933902) at 0.1 µg/mL. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • Poly23A05_PURE_EGFR_Antibody_1_120524
    Whole cell extracts (15 µg total protein) from A-431 cells either untreated (-) or treated (+) with EGF (Cat. No. 585506) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL of Purified anti-EGFR Phospho (Tyr1045) (clone Poly23A05) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP Donkey anti-rabbit IgG (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Total EGFR loading was confirmed using Purified anti-EGFR (clone A19002A) (Cat. No. 933902) at 0.1 µg/mL. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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637151 50 µL 256€
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637152 200 µL 636€
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Description

Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that links extracellular mitogenic ligand binding to complex downstream signaling cascades. Initial ligand binding results in receptor oligomerization and autophosphorylation of multiple tyrosine residues within cytosolic domains of the protein. Phosphorylation at tyrosine 1045 allows for binding directly to the adaptor protein GRB2. EGFR stimulation leads to activation of RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCγ-PKC and STAT signaling cascades, and constitutive activation of the receptor promotes tumorigenesis in multiple cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Synthetic peptide corresponding to human EGFR phosphorylated at tyrosine 1045
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution of this reagent is 1:400 - 1:4000. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody (clone Poly23A05) is intended as a replacement for another antibody against the same target (clone Poly6563). Poly23A05 has a higher affinity for the target protein than Poly6563. 

RRID
AB_3097617 (BioLegend Cat. No. 637151)
AB_3097617 (BioLegend Cat. No. 637152)

Antigen Details

Structure
EGFR is a 1,210 amino acid protein with a predicted molecular weight of 134 kD; the processed form is 1,186 amino acids in length.
Distribution

Plasma membrane

Function
EGFR signaling, cell signaling
Interaction
GRB2, SOS1, STAT1, STAT3, JAK2, CRK
Ligand/Receptor
Epidermal growth factor (EGF)
Cell Type
Epithelial cells
Biology Area
Cell Biology, Cell Proliferation and Viability, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Hunter T and Cooper JA. 1981. Cell. 24:741-52.
  2. Gill GN and Lazar CS. 1981. Nature. 293:305-7.
  3. Reynolds FH, et al. 1981. Nature. 292:259-62.
  4. Biscardi JS, et al. 1999. J Biol Chem. 274:8335-42.
  5. Zhou M, et al. 2013. Cancer Res. 23:7056-67-67.
Gene ID
1956 View all products for this Gene ID
UniProt
View information about EGFR on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/03/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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