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- Clone
- 6F8-D9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Histone H4, histone 1, H4a, H4 histone family, member A, H4K20me3
- Previously
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Covance Catalog# MMS-5288
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
IHC staining of purified anti-Histone H4 Trimethyl (Lys 20) antibody (clone 6F8-D9) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. -
Western blot of purified anti-Histone H4 Trimethyl (Lys 20) antibody (clone 6F8-D9). Lane M: Molecular weight marker; Lane 1: 15 µg of Jurkat cell lysate; Lane 2: 15 µg of RAW264.7 cell lysate; Lane 3: 15 µg of UMR106 cell lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control (bottom blot). The bands were visualized using chemiluminescence detection.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 827701 | 100 µL | 220€ | ||||
Histone proteins are classified into core histones (H2A, H2B, H3, H4) and linker histones (H1, H5). Core histones form an octamer, which contains two H2A-H2B dimers and one H3-H4 tetramer. Core histones are predominantly globular except for the unstructured N-terminal tails. Posttranslational modifications, such as acetylation, methylation, phosphorylation, ubiquitination, SUMOylation and ADP-ribosylation occur in histone tails.
Histone modifications induce changes of chromatin structure and thereby affect the accessibility of transcription factors, nuclear proteins and enzymes to genomic DNA, resulting in gene activation or repression. It is known that histone modifications play critical roles in DNA repair, DNA replication, transcription regulation, alternative splicing and chromosome condensation and some diseases including autoimmune diseases and cancers.
Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a synthetic peptide conjugated to KLH containing trimethylated lysine 20 of human Histone H4
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/mL
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
IHC-P - Quality tested
WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.0 - 10 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
-
- Botuyan MV, et al. 2006. Cell. 127:1361. (WB)
- Product Citations
-
- RRID
-
AB_2564914 (BioLegend Cat. No. 827701)
Antigen Details
- Structure
- Histone proteins H3 and H4 bind to form a tetramer. This tetramer further combines with two H2a-H2b dimers to form the compact Histone octamer core.
- Distribution
-
Histone H4 is ubiquitously expressed in all cells.
- Function
- Transcription regulation, DNA repair, DNA replication, chromosomal stability, silencing of repetitive DNA and transposons.
- Interaction
- Various transcription factors.
- Biology Area
- Cancer Biomarkers, Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
- Antigen References
-
1. Chen HM, et al. 2015. Epigenetics and Dermatology 409.
2. Bhasin M, et al. 2006. J. Comput. Biol. 13:102.
3. Nelson DM, et al. 2016. Genome Biol. 17:158. - Gene ID
- 8359 View all products for this Gene ID
- UniProt
- View information about Histone on UniProt.org
Related FAQs
Other Formats
View All Histone Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Histone H4 Trimethyl (Lys 20) | 6F8-D9 | IHC-P,WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Histone H4 Trimethyl (Lys 20)
IHC staining of purified anti-Histone H4 Trimethyl (Lys 20) ... 
Western blot of purified anti-Histone H4 Trimethyl (Lys 20) ...
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