Purified anti-human CD197 CCR7 Maxpar Ready Antibody anti-CD197

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Clone: G043H7 (See other available formats)
Regulatory Status: RUO
Other Names: BLR2, CDw197, EBI1, CMKBR7
Isotype: Mouse IgG2a, κ
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Product Citations: publications

Human PBMCs stained with 170Er-anti-CD3 (UCHT1) and 159Tb-anti-CD197 (G043H7). Lymphocytes are displayed in the analysis. Data provided by DVS Sciences.

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353237

100 µg

156€

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Description

CCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity by affecting interactions between T cells and dendritic cells and their downstream effect. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B cells recirculate between B-cell-rich compartments (follicles or B cell zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CCR7-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
RRID: AB_2563726 (BioLegend Cat. No. 353237)

Antigen Details

Structure: Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.
Distribution: T cells, B cells, NK, dendritic cells.
Function: The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.
Ligand/Receptor: CCL19 and CCL21.
Cell Type: B cells, Dendritic cells, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Yanagihara S, et al. 1998. J. Immunol. 161:3096.
2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610.
3. Reif K, et al. 2002. Nature 416:94.
4. Nakata B, et al. 2008. Oncology 74:69.
5. Brodie T. et al. 2013. Cytometry A. 6: 530-2. PubMed
6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9 PubMed
7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8 PubMed
Gene ID: 1236 View all products for this Gene ID
UniProt: View information about CD197 on UniProt.org

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Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD197 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD197 (CCR7)	G043H7	FC
Alexa Fluor® 488 anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 421™ anti-human CD197 (CCR7)	G043H7	FC
PE anti-human CD197 (CCR7)	G043H7	FC
APC/Cyanine7 anti-human CD197 (CCR7)	G043H7	FC
Pacific Blue™ anti-human CD197 (CCR7)	G043H7	FC
APC anti-human CD197 (CCR7)	G043H7	FC
FITC anti-human CD197 (CCR7)	G043H7	FC
Alexa Fluor® 647 anti-human CD197 (CCR7)	G043H7	FC
PerCP/Cyanine5.5 anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 605™ anti-human CD197 (CCR7)	G043H7	FC
PE/Cyanine7 anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 711™ anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 785™ anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 510™ anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 650™ anti-human CD197 (CCR7)	G043H7	FC
PE/Dazzle™ 594 anti-human CD197 (CCR7)	G043H7	FC
Biotin anti-human CD197 (CCR7)	G043H7	FC
Purified anti-human CD197 (CCR7) (Maxpar® Ready)	G043H7	FC,CyTOF®
PerCP anti-human CD197 (CCR7)	G043H7	FC
Alexa Fluor® 700 anti-human CD197 (CCR7)	G043H7	FC
APC/Fire™ 750 anti-human CD197 (CCR7)	G043H7	FC
TotalSeq™-A0148 anti-human CD197 (CCR7)	G043H7	PG
TotalSeq™-B0148 anti-human CD197 (CCR7)	G043H7	PG
TotalSeq™-C0148 anti-human CD197 (CCR7)	G043H7	PG
Brilliant Violet 750™ anti-human CD197 (CCR7)	G043H7	FC
Ultra-LEAF™ Purified anti-human CD197 (CCR7)	G043H7	FC,Block
Spark NIR™ 685 anti-human CD197 (CCR7)	G043H7	FC
KIRAVIA Blue 520™ anti-human CD197 (CCR7)	G043H7	FC
PE/Fire™ 640 anti-human CD197 (CCR7)	G043H7	FC
Spark YG™ 581 anti-human CD197 (CCR7)	G043H7	FC
APC/Fire™ 810 anti-human CD197 (CCR7) Antibody	G043H7	FC
TotalSeq™-D0148 anti-human CD197 (CCR7)	G043H7	PG
PE/Fire™ 810 anti-human CD197 (CCR7) Antibody	G043H7	FC
PE/Cyanine5 anti-human CD197 (CCR7)	G043H7	FC
Spark YG™ 593 anti-human CD197 (CCR7)	G043H7	FC
PE/Fire™ 744 anti-human CD197 (CCR7)	G043H7	FC
Spark Red™ 718 anti-human CD197 (CCR7) (Flexi-Fluor™)	G043H7	FC
Spark Red™ 718 anti-human CD197 (CCR7)	G043H7	FC
PE anti-human CD197	G043H7	FC
Spark PLUS UV395™ anti-human CD197 (CCR7)	G043H7	FC
PerCP/Fire™ 780 anti-human CD197 (CCR7) Antibody	G043H7	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 07/22/2022

For Research Use Only. Not for diagnostic or therapeutic use.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.