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Purified anti-human/mouse SSEA-3 Antibody

Pricing & Availability
Clone
MC-631 (See other available formats)
Regulatory Status
RUO
Other Names
Stage-Specific Embryonic Antigen-3
Isotype
Rat IgM, κ
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Product Citations
publications
MC-631_Pure_090808.jpg
NCCIT, human teratocarcinoma cell line stained with purified MC-631, followed by anti-rat Ig FITC
  • MC-631_Pure_090808.jpg
    NCCIT, human teratocarcinoma cell line stained with purified MC-631, followed by anti-rat Ig FITC
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330302 100 µg 216€
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Description

Stage-Specific Embryonic Antigen-3 (SSEA-3) is an internal core structure of glob-series glycolipids (R-Galβ1-3GalNAcβ1-3Galα1-4Galβ1-R). SSEA-3 is expressed on human teratocarcinoma cells (embryonal carcinoma, EC), embryonic stem cells (ESC), embryonic germ cells (EG), and preimplantation embryos. The expression of SSEA-3 on human ESC is downregulated upon differentiation. SSEA-3 is also expressed by mouse on unfertilized eggs and preimplantation to early cleaved embryos.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Reported Reactivity
African Green
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
4-8 cell stage mouse embryos
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1.

Application References
  1. Kannagi R, et al. 1983. EMBO 2:2355. (IF)
  2. Kaufmann B, et al. 2005. Virology 332:189. (ELISA)
Product Citations
  1. Wakao S, et al. 2022. Cell Mol Life Sci. 79:542. PubMed
  2. Oguma Y, et al. 2022. iScience. 25:105395. PubMed
  3. Hey CAB et al. 2018. Stem Cell Res. 33:46-50 . PubMed
  4. Hori Y, et al. 2022. Front Cardiovasc Med. 9:981088. PubMed
  5. Hey CAB et al. 2018. Stem cell research. 31:235-239 . PubMed
  6. Fujita Y, et al. 2021. J Invest Dermatol. 198:141. PubMed
  7. Frederiksen HR et al. 2019. Stem cell research. 1725:03:00 . PubMed
  8. Frederiksen HR et al. 2019. Stem cell research. 1723:28:00 . PubMed
  9. Schmid B et al. 2019. Stem Cell Res. 1723:09:00 . PubMed
RRID
AB_1236554 (BioLegend Cat. No. 330302)

Antigen Details

Distribution

Embryonic stemcells

Cell Type
Embryonic Stem Cells
Biology Area
Cell Biology, Immunology, Stem Cells
Gene ID
93961 View all products for this Gene ID
UniProt
View information about SSEA-3 on UniProt.org
Go To Top Version: 4    Revision Date: 05/07/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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