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Purified anti-human Notch 1 Antibody

Pricing & Availability
Clone
MHN1-519 (See other available formats)
Regulatory Status
RUO
Other Names
Neurogenic locus notch homolog protein 1 (Notch 1), Translocation-associated notch protein (TAN-1), Motch A, mT14, p300
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
MHN1-519_PE_1_051911
PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with Notch 1 (clone MHN1-519) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • MHN1-519_PE_1_051911
    PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with Notch 1 (clone MHN1-519) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • MHN1-519_PE_2_051911
    Human Notch 1 transfected CHO cells were stained with Notch 1 (clone MHN1-519) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
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352102 100 µg 137€
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Description

Notch 1, also known as TAN-1, is a transmembrane protein. Its extracellular domain contains 29 epidermal growth factor-like (EGF) repeats and 3 Lin/Notch Glp (LNR) repeats, the intracellular domain contains 5 CDC10/Ankryn repeats (ANK), 1 proline, glutamate, serine, threonine-rich (PEST) motif, and 1 regulation of amino acid metabolism 23 (RAM23) domain. Notch 1 regulates the development, differentiation, and survival of a broad spectrum of cell lineages. It is involved in  myogenesis, neurogenesis, gliogenesis, and lymphocyte development, resulting in Notch 1 expression in many organs such as brain, lung, thymus, spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney, and heart. Notch 1 ligands are Jagged 1, Jagged 2, Delta 1, and Delta 4. Upon ligand binding, the intracellular domain of Notch 1 is cleaved and translocates to the cell nucleus where it forms a transcriptional activator complex with RBP-J κ.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Notch1-Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blocking Notch 1 mediated binding to DLL4 in human cord blood CD34+ cells1.

Application References
  1. Haraguchi K, et al. 2009. J. Immunol. 182:6168. (Block)
  2. Yamanda S, et al. 2009. Blood 113:3631. (FC)
  3. Guy CS. et al. 2013. Nat Immunol. 14:262. PubMed
Product Citations
  1. Baran N, et al. 2022. Nat Commun. 13:2801. PubMed
  2. Guy C, et al. 2013. Nat Immunol. 14:262. PubMed
RRID
AB_10901001 (BioLegend Cat. No. 352102)

Antigen Details

Structure
Transmembrane protein. The extracellular domain contains 29 EGF repeats and 3 LNR repeats. The intracellular domain contains 5 CDC10/ANK, 1 PEST motif, and 1 RAM23 domain.
Distribution

Highly expressed in the brain, lung, and thymus. Lower levels of expression in spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney, and heart.

Function
Regulates development, differentiation, and survival of a broad spectrum of cell lineages. Involved in myogenesis, neurogenesis, gliogenesis, and lymphocyte development.
Interaction
RBP-J κ.
Ligand/Receptor
Jagged 1, Jagged 2, Delta 1, Delta 4.
Cell Type
B cells, Neural Stem Cells, Thymocytes
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family
Postsynaptic proteins
Antigen References

1. Vicente R, et al. 2010. Semin. Immunol. 22:270.
2. Zhao WL. 2010. Leukemia 24:13.
3. Sanda T, et al. 2010. Blood 115:1735.
4. Zhou J, et al. 2009. Immunity 31:356.

Gene ID
4851 View all products for this Gene ID
UniProt
View information about Notch 1 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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