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Purified anti-HuR (ELAVL1) Antibody

Pricing & Availability
Clone
W18273D (See other available formats)
Regulatory Status
RUO
Other Names
ELAV Like RNA Binding Protein 1, ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R), Embryonic Lethal, Abnormal Vision, Drosophilia, Homolog-Like 1, ELAV-Like Protein 1, Hu Antigen R, MeIG, Hua,
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18273D_PURE_HuR_Antibody_1_110220
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-HuR (ELAVL1) antibody (clone W18273D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker.
  • W18273D_PURE_HuR_Antibody_1_110220
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-HuR (ELAVL1) antibody (clone W18273D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker.
  • W18273D_PURE_HuR_Antibody_2_110220
    Untreated HeLa cells (panel A) or HeLa cells treated with 5.0 µg/mL Actinomysin D for 3 hours (panel B) were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 1.0 µg/mL (1:500 dilution) purified anti-HuR (ELAVL1) antibody (clone W18273D) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
  • W18273D_PURE_HuR_Antibody_3_110220
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg of purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400502) or purified anti-HuR (ELAVL1) antibody (clone W18273D). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a rabbit control antibody against a separate epitope of HuR. Lane M: Molecular weight marker.
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941601 25 µg 95€
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941602 100 µg 235€
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Description

Adenylate-uridylate-rich elements, or AREs, are short regions of RNA found in the 3’ UTR of messenger RNA. AREs are a major determinant of mRNA stability and translational efficiency, and play a crucial role in the post-transcriptional regulation of genes involved in several cellular processes.

In humans, the Hu family of proteins is comprised of 4 members, each of which stabilize ARE-containing mRNA through directly binding to the ARE motif. In contrast to the Hu family members HuB, HuC, and HuD, which show neural specific distribution, HuR (also known as ELAVL1) is expressed in all tissues. While HuR is largely a resident nuclear protein under normal conditions, in response to cellular stress it can chaperone mRNA from the nucleus to the cytosol and continue to stabilize mRNA once in the cytosol.

HuR is overexpressed and cytosolically delocalized in a wide range of cancers. In in vitro studies, HuR has been shown to stabilize several ARE-harboring mRNA encoding proteins involved in tumorigenesis and transformation, including MMP9, Bax, Fos, p21, and multiple cyclins, and is promising therapeutic target for cancer therapy.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human HuR protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC using 4% PFA-fixed HeLa cells permeabilized with either methanol or Triton X-100. While staining was observed with both methods, methanol permeabilization resulted in stronger HuR staining.

RRID
AB_2888897 (BioLegend Cat. No. 941601)
AB_2888897 (BioLegend Cat. No. 941602)

Antigen Details

Structure
HuR is a 326 amino acid protein with a predicted molecular weight of 36 kD.
Distribution

Ubiquitously expressed/Nucleus, cytosol, and stress granules

Function
mRNA stability, mRNA nuclear export/post-transcriptional gene regulation, embryonic development
Biology Area
Cell Biology
Antigen References
  1. Peng SS, et al. 1998. EMBO J. 17:3461-70.
  2. Fan XC & Steitz JA. 1998. Proc Natl Acad Sci U S A.  95:15293-8.
  3. Wang W, et al. 2002. Mol Cell Biol. 22:3425-36.
Gene ID
1994 View all products for this Gene ID
UniProt
View information about HuR on UniProt.org
Go To Top Version: 1    Revision Date: 11/02/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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