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Purified anti-Keratin 5 Polyclonal Chicken Antibody

Pricing & Availability
Clone
Poly9059 (See other available formats)
Regulatory Status
RUO
Other Names
Keratin, type II cytoskeletal 5, CK-5, 58 kD cytokeratin, cytokeratin-5, type-II keratin Kb5, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, keratin 5 (epidermolysis bullosa simplex), krt5
Isotype
Chicken IgY
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Product Citations
publications
Poly9059_PURE_Keratin5_antibody_102517
IHC staining of anti-Keratin 5 antibody (Poly90590) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Sodium Citrate (Cat. No. 928602), the tissue was incubated with the primary antibody for 60 minutes at room temperature at 1 µg/ml. After incubation with biotinylated anti-chicken antibody, the tissue was labeled with BioLegend’s HRP labeling reagent followed by addition of AEC chromogen (Cat. No. 925804). The tissue was then counterstained with hematoxylin.
  • Poly9059_PURE_Keratin5_antibody_102517
    IHC staining of anti-Keratin 5 antibody (Poly90590) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Sodium Citrate (Cat. No. 928602), the tissue was incubated with the primary antibody for 60 minutes at room temperature at 1 µg/ml. After incubation with biotinylated anti-chicken antibody, the tissue was labeled with BioLegend’s HRP labeling reagent followed by addition of AEC chromogen (Cat. No. 925804). The tissue was then counterstained with hematoxylin.
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905903 25 µg 172€
 
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905904 100 µg 286€
 
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Description

Keratin 5 belongs to the type II cytokeratin family of proteins. It dimerizes with Keratin 14 to form intermediate filaments (IFs) that make up the cytoskeleton of the basal epithelial cells. Keratin 5 is involved in several diseases including breast and lung cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Polyclonal
Host Species
Chicken
Immunogen
This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse keratin 5 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 – 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Wei X, et al. 2022. Cell Rep. 40:111313. PubMed
  2. Song H, et al. 2023. Dev Dyn. 252:527. PubMed
  3. Tsuji S, et al. 2022. Nat Aging. 2:115. PubMed
  4. Saraswathula A, et al. 2023. Int Forum Allergy Rhinol. 13:230. PubMed
  5. Belardin LB, et al. 2022. Andrology. 10:1522. PubMed
  6. Lyu H, et al. 2022. Cell Rep. 41:111863. PubMed
  7. Yan Y, et al. 2023. Cancers (Basel). 15: . PubMed
  8. Chalmers FE, et al. 2022. Dev Biol. 485:9. PubMed
  9. Grbesa I, et al. 2021. Cell Rep. 36:109625. PubMed
  10. Carraro G, et al. 2020. Am J Respir Crit Care Med. 202:1540. PubMed
  11. Paris AJ, et al. 2020. Nat Cell Biol. 1.747916667. PubMed
  12. Eshiba S, et al. 2021. Cell Reports. 36(5):109492. PubMed
  13. van Ineveld RL, et al. 2022. Nat Protoc. 17:3028. PubMed
  14. Yang K, et al. 2022. J Clin Invest. 132:. PubMed
  15. Dong B, et al. 2020. Commun Biol. 3:778. PubMed
  16. Kadur Lakshminarasimha Murthy P, et al. 2022. Nature. 604:111. PubMed
  17. Keshvari S, et al. 2021. PLoS Genet. 17:e1009605. PubMed
  18. Nanba D, et al. 2021. J Cell Biol. 220:. PubMed
  19. Wei JP, et al. 2020. Exp Dermatol. 29:400. PubMed
  20. Yuan T, et al. 2020. Front Pharmacol. 11:120. PubMed
  21. Yoon YJ, et al. 2022. Nat Commun. 13:3291. PubMed
  22. Liang Z, et al. 2022. iScience. 25:105233. PubMed
RRID
AB_2721742 (BioLegend Cat. No. 905903)
AB_2721742 (BioLegend Cat. No. 905904)

Antigen Details

Structure
Keratin 5 is a 590 amino acid protein with a molecular mass of 62 kD.
Distribution

Tissue distribution: esophagus, hair follicle, saliva, and skin.
Cellular distribution: cytoskeleton, extracellular, nucleus, and cytosol.

Function
Keratin 5 IFs contribute to the cell architecture and provide the cells with elasticity to withstand mechanical and non-mechanical stresses.
Interaction
Keratin 14
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References
  1. Yuspa SH, et al 1989 J Cell Biol 109:1207.
  2. Roop, DR, et al 1984 J Biol Chem 259:8037.
Gene ID
3852 View all products for this Gene ID
UniProt
View information about Keratin 5 on UniProt.org

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Go To Top Version: 2    Revision Date: 09/25/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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