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Purified anti-LEF1 Antibody

Pricing & Availability
Clone
15H5A18 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha (TCF1-alpha)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_15H5A18_PURE_LEF1_Antibody_WB_121217
Total lysates (15 µg protein) from U87-MG (Low expression negative control) and Jurkat (Positive control) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/mL) purified anti-LEF1 (clone 15H5A18). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 1_15H5A18_PURE_LEF1_Antibody_WB_121217
    Total lysates (15 µg protein) from U87-MG (Low expression negative control) and Jurkat (Positive control) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/mL) purified anti-LEF1 (clone 15H5A18). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 2_15H5A18_PURE_LEF1_Antibody_2_IP_060716
    Immunoprecipitation of LEF1 from Jurkat cell extracts. Lane 1 is 5% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-LEF1 antibody (clone 15H5A18, lane 4). Western blot was performed using anti-LEF1 antibody (clone 15H5A18).
  • 3_15H5A18_PURE_LEF1_Antibody_3_ICFC_092523
    Jurkat cells (positive control, filled histogram) and Daudi cells (negative control, open histogram) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with purified anti-LEF1 (clone 15H5A18), or purified mouse IgG1, κ isotype control (Cat. No. 401402) (open histogram, dashed line) followed by PE goat anti-mouse IgG (Cat. No. 405307).
Cat # Size Price Quantity Check Availability Save
653101 25 µg 81€
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653102 100 µg 203€
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Description

Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor belonging to the TCF/LEF family. LEF1 was first identified as a protein binding to TCR-α enhancer and upregulates expression of T-cell antigen receptor α chain. LEF1 is involved in Wnt signaling pathways as a ubiquitous regulator. During Wnt signaling, translocation of β-catenin from the cytosol to the nucleus results in elevation of transcriptional activity of LEF1, which subsequently regulates multiple downstream genes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human LEF1 recombinant protein (1-298 aa)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Concentration
0.5 mg/mL
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per mL (1:5000 - 1:500). For immunoprecipitation, the suggested use of this reagent is 5 - 20 μg per mL. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

LEF-1 is known to have 7 isoforms.

IHC-P (Immunohistochemistry FFPE) is not suggested for this product.

For ICFC (intracellular flowcytometry), True-Phos™ Perm Buffer (Cat. No. 425401) is suggested for permeabilization.

Product Citations
  1. Duraiswamy J, et al. 2021. Cancer Cell. 39:1623. PubMed
RRID
AB_2561614 (BioLegend Cat. No. 653101)
AB_2561614 (BioLegend Cat. No. 653102)

Antigen Details

Structure
44 kD protein containing a DNA-binding domain (HMG box), an CTNNB1-binding region, and a Prolin-rich domain
Distribution

Nucleus

Function
LEF1 is a transcription factor mediating canonical Wnt signaling pathway. It has been shown to function in hair bud formation, cancer development, tumorigenesis, angiogenesis, and T-cell maturation.
Interaction
Interacts with ALX4, AML-1, beta-catenin (CTNNB1), EP300, MITF, PIAS4, SMAD2, and SMAD3
Biology Area
Angiogenesis, Cell Biology, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers, TCRs
Antigen References
  1. Mallory MJ, et al. 2011. Mol. Cell. Biol. 31:2184.
  2. Waterman ML, et al. 1991. Genes Dev. 5:656.
  3. Hovanes K, et al. 2001. Nat Genet. 28:53.
  4. Bruhn L, et al. 1997. Genes Dev. 11:640.
  5. Nguyen A, et al. 2005. Int. J. Oncol. 27:949.
  6. Petropoulos K, et al. 2008. J. Exp. Med. 205:515.
Gene ID
51176 View all products for this Gene ID
UniProt
View information about LEF1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 7    Revision Date: 02/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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