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Purified anti-LRRK2 Antibody (Previously Covance catalog# MMS-5301)

Pricing & Availability
Clone
8G10 (See other available formats)
Regulatory Status
RUO
Other Names
Leucine-rich repeat serine/threonine-protein kinase 2, augmented in rheumatoid arthritis 17, AURA17, DARDARIN, PARK8, RIPK7, ROCO2
Previously
Covance Catalog# MMS-5301
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
8G10_Purified_LRRK2_Antibody_WB_083118
Western blot of purified anti-LRRK2 antibody (clone 8G10). Lane 1: Molecular weight marker; Lane 2: 30 µg of HEK293 cell lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 8G10_Purified_LRRK2_Antibody_WB_083118
    Western blot of purified anti-LRRK2 antibody (clone 8G10). Lane 1: Molecular weight marker; Lane 2: 30 µg of HEK293 cell lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Check Availability Save
844402 25 µL 85€
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844401 100 µL 220€
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Description

Leucine-rich repeat kinase 2 (LRRK2), is a member of the leucine-rich repeat kinase family. This protein also functions as a GTPase. Proteins with leucine-rich regions play a role in protein-protein interactions and mediate assembly of the cell's structural framework (cytoskeleton). Mutations in LRRK2 genes have been associated with late-onset Parkinson's disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Antibody is against amino acids 100 - 500 of human LRRK2.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Wang X, et al. 2021. Sci Rep. 11:12900. PubMed
RRID
AB_2565614 (BioLegend Cat. No. 844402)
AB_2565614 (BioLegend Cat. No. 844401)

Antigen Details

Structure
This gene is a member of the leucine-rich repeat kinase family and is encoded by the PARK8 gene. The protein is present mainly in the cytoplasm but can be associated with the mitochondrial outer membrane.
Expected MW: >200 kD
Distribution

Tissue distribution: Lung, Kidney, parathyroid gland, CNS
Cellular distribution: cytoplasm, mitochondrion, nucleus, endoplasmic reticulum, lysosome, Golgi apparatus, endosome

Function
LRRK2 interacts with the C-terminal R2 RING finger domain of parkin. LRRK2 positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. Mutations in this gene have been associated with Parkinson disease.
Biology Area
Cell Biology, Mitochondrial Function, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
Mitochondrial Markers
Antigen References
  1. Paisán-Ruíz C, et al. 2004. Neuron. 44(4):595-600.
  2. Alegre-Abarrategui J, et al. 2009. Hum. Mol. Genet. 18(21):4022-34.
  3. Gómez-Suaga P, et al. 2012. Hum. Mol. Genet. 21(3):511–25.
  4. Orenstein SJ, et al. 2013. Nat. Neurosci. 16(4):394–406.
  5. Cherra SJ, et al. 2013. Am. J. Pathol. 182(2):474–84. 
Gene ID
120892 View all products for this Gene ID
UniProt
View information about LRRK2 on UniProt.org
Go To Top Version: 5    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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